Incorporation of doxorubicin into plant-derived nanovesicles: process monitoring and activity assessment.

IF 6.5 2区医学 Q1 PHARMACOLOGY & PHARMACY Drug Delivery Pub Date : 2025-12-01 Epub Date: 2024-12-11 DOI:10.1080/10717544.2024.2439272

Aleksandra Steć, Monika Targońska, Shishir Jaikishan, Rui Chen, Piotr Mucha, Grzegorz S Czyrski, Jacek Jasiecki, Agata Płoska, Andrea Heinz, Susanne K Wiedmer, Leszek Kalinowski, Krzysztof Waleron, Bartosz Wielgomas, Szymon Dziomba

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Abstract

Extracellular vesicles (EVs) are an experimental class of drug carriers. Alternative sources of EVs are currently being explored to overcome limitations related to their manufacturing from mesenchymal stem cells. In this work, Citrus limon-derived EVs were tested as carriers for the widely used chemotherapeutic drug - doxorubicin (DOX). Capillary electrophoresis (CE) and nanoplasmonic sensing (NPS) were developed for the quality control of DOX-EV preparations. It was found that the CE method enables simultaneous detection of free and incorporated DOX and allows assessing the stability of the preparations and the drug leakage. NPS, on the other hand, demonstrated that DOX is accumulated in the interfacial region of the carrier. The activity of DOX-loaded EVs was tested on HeLa (cervical cancer cells) and HEK293T (human embryonic kidney cells) cell lines. It was found that DOX incorporation into plant-derived EVs virtually does not affect the drug's cytotoxicity to HeLa cells but significantly decreases DOX activity against HEK293T cell line.

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阿霉素并入植物源性纳米囊泡：过程监测和活性评估。

细胞外囊泡（EVs）是一类实验性的药物载体。目前正在探索电动汽车的替代来源，以克服与制造间充质干细胞有关的限制。在这项工作中，柑橘柠檬衍生的电动汽车作为广泛使用的化疗药物-阿霉素（DOX）的载体进行了测试。建立了毛细管电泳（CE）和纳米等离子体传感（NPS）技术用于DOX-EV制剂的质量控制。发现CE方法可以同时检测游离DOX和掺入DOX，并可以评估制剂的稳定性和药物泄漏。另一方面，NPS表明DOX积聚在载体的界面区域。在HeLa（宫颈癌细胞）和HEK293T（人胚胎肾细胞）细胞系上检测载dox ev的活性。研究发现，DOX掺入植物源性ev几乎不影响药物对HeLa细胞的细胞毒性，但会显著降低DOX对HEK293T细胞系的活性。

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来源期刊

Drug Delivery 医学-药学

CiteScore

11.80

自引率

5.00%

发文量

250

审稿时长

3.3 months

期刊介绍： Drug Delivery is an open access journal serving the academic and industrial communities with peer reviewed coverage of basic research, development, and application principles of drug delivery and targeting at molecular, cellular, and higher levels. Topics covered include all delivery systems including oral, pulmonary, nasal, parenteral and transdermal, and modes of entry such as controlled release systems; microcapsules, liposomes, vesicles, and macromolecular conjugates; antibody targeting; protein/peptide delivery; DNA, oligonucleotide and siRNA delivery. Papers on drug dosage forms and their optimization will not be considered unless they directly relate to the original drug delivery issues. Published articles present original research and critical reviews.