miR-6760-5p suppresses neoangiogenesis by targeting Yes-associated protein 1 in patients with moyamoya disease undergoing indirect revascularization

IF 2.6 3区生物学 Q2 GENETICS & HEREDITY Gene Pub Date : 2024-12-09 DOI:10.1016/j.gene.2024.149152

Yunyu Wen , Junda Chen , Tinghan Long , Fangzhou Chen , Zhibin Wang , Siyuan Chen , Guozhong Zhang , Mingzhou Li , Shichao Zhang , Huibin Kang , Wenfeng Feng , Gang Wang

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Abstract

Objective

The aim of this research was to investigate the specific regulatory role of miR-6760-5p in angiogenesis in moyamoya disease.

Methods

HUVECs were transfected with miR-6760-5p inhibitor and mimics fragments, then subjected to assays for cell proliferation, migration, and tube formation. Subsequently, downstream target genes of miR-6760-5p were predicted and the protein expression levels of these genes were evaluated. The presence of miR-6760-5p and YAP1 was verified by a dual luciferase reporter gene test, followed by an assessment of the effects of YAP1 and miR-6760-5p on the HUVECs.

Results

Comparatively to the control group, increased expression of miR-6760-5p decreased cell growth, movement, and tube formation. YAP1 gene was discovered as a target controlled by miR-6760-5p, with subsequent investigation confirming YAP1 as a gene regulated by miR-6760-5p. Additionally, miR-6760-5p was found to counteract the angiogenesis-promoting effect of YAP1.

Conclusion

The results of this research suggest a possible link between the miR-6760-5p gene found in the cerebrospinal fluid of individuals with moyamoya disease and the process of vascularization in this particular condition. The findings indicate that miR-6760-5p may be a new molecular indicator and potential target for the diagnosis of moyamoya disease.

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miR-6760-5p通过靶向烟雾病患者间接血运重建术中的yes相关蛋白1抑制新血管生成。

目的：本研究旨在探讨miR-6760-5p在烟雾病血管生成中的具体调控作用。方法：用miR-6760-5p抑制剂和模拟物片段转染HUVECs，然后进行细胞增殖、迁移和管形成的检测。随后，预测miR-6760-5p的下游靶基因，并评估这些基因的蛋白表达水平。通过双荧光素酶报告基因测试验证了miR-6760-5p和YAP1的存在，随后评估了YAP1和miR-6760-5p对huvec的影响。结果：与对照组相比，miR-6760-5p表达增加可降低细胞生长、运动和成管。YAP1基因被发现是受miR-6760-5p调控的靶标，随后的研究证实YAP1是受miR-6760-5p调控的基因。此外，miR-6760-5p被发现可以抵消YAP1促进血管生成的作用。结论：本研究结果提示，烟雾病患者脑脊液中发现的miR-6760-5p基因与这种特殊情况下的血管化过程可能存在联系。提示miR-6760-5p可能成为烟雾病诊断的新分子指标和潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.