Structures of PSI-FCPI from Thalassiosira pseudonana grown under high light provide evidence for convergent evolution and light-adaptive strategies in diatom FCPIs.

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Integrative Plant Biology Pub Date : 2024-12-13 DOI:10.1111/jipb.13816
Yue Feng, Zhenhua Li, Yang Yang, Lili Shen, Xiaoyi Li, Xueyang Liu, Xiaofei Zhang, Jinyang Zhang, Fei Ren, Yuan Wang, Cheng Liu, Guangye Han, Xuchu Wang, Tingyun Kuang, Jian-Ren Shen, Wenda Wang
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Abstract

Diatoms rely on fucoxanthin chlorophyll a/c-binding proteins (FCPs) for light harvesting and energy quenching under marine environments. Here we report two cryo-electron microscopic structures of photosystem I (PSI) with either 13 or five fucoxanthin chlorophyll a/c-binding protein Is (FCPIs) at 2.78 and 3.20 Å resolutions from Thalassiosira pseudonana grown under high light (HL) conditions. Among them, five FCPIs are stably associated with the PSI core, these include Lhcr3, RedCAP, Lhcq8, Lhcf10, and FCP3. The eight additional Lhcr-type FCPIs are loosely associated with the PSI core and detached under the present purification conditions. The pigments of this centric diatom showed a higher proportion of chlorophylls a, diadinoxanthins, and diatoxanthins; some of the chlorophyll as and diadinoxanthins occupy the locations of fucoxanthins found in the huge PSI-FCPI from another centric diatom Chaetoceros gracilis grown under low-light conditions. These additional chlorophyll as may form more energy transfer pathways and additional diadinoxanthins may form more energy dissipation sites relying on the diadinoxanthin-diatoxanthin cycle. These results reveal the assembly mechanism of FCPIs and corresponding light-adaptive strategies of T. pseudonana PSI-FCPI, as well as the convergent evolution of the diatom PSI-FCPI structures.

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硅藻在海洋环境中依靠叶绿素a/c结合蛋白(FCPs)进行光收集和能量淬灭。这里我们报告了生长在强光(HL)条件下的Thalassiosira pseudonana光合系统I(PSI)的两种冷冻电镜结构,分辨率分别为2.78和3.20埃,其中有13个或5个岩藻黄素叶绿素a/c结合蛋白Is(FCPIs)。其中,5 个 FCPIs 与 PSI 核心有稳定的关联,包括 Lhcr3、RedCAP、Lhcq8、Lhcf10 和 FCP3。另外 8 个 Lhcr 型 FCPI 与 PSI 核心结合松散,在目前的纯化条件下会分离。这种向心硅藻的色素中叶绿素 a、二二氧黄素和二二氧黄素的比例较高;其中一些叶绿素 as 和二二氧黄素占据了另一种在弱光条件下生长的向心硅藻 Chaetoceros gracilis 的巨大 PSI-FCPI 中的岩藻黄素的位置。这些额外的叶绿素可能会形成更多的能量转移途径,而额外的二齿黄素可能会依靠二齿黄素-二齿黄素循环形成更多的能量耗散位点。这些结果揭示了假褐藻 PSI-FCPI 的 FCPIs 组装机制和相应的光适应策略,以及硅藻 PSI-FCPI 结构的趋同进化。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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