Spatial chromosome organization and adaptation of the radiation-resistant extremophile Deinococcus radiodurans.

Abstract

Radiation-resistant Deinococcus radiodurans is an extremophilic microorganism capable of withstanding high levels of ionizing radiation and chemical mutagens. It possesses remarkable DNA repair capability and serves as a model organism for studying stress resistance mechanisms. However, our understanding of the spatial chromosome organization of this species remains limited. In this study, we employed chromosome conformation capture (3C) technology to determine the 3D genome structure of D. radiodurans and to further investigate the changes of chromosome conformation induced by ultraviolet (UV) irradiation. We observed that UV irradiation reduced short-range chromosome interactions, and smaller chromosomal interaction domains (CIDs) merged to form larger CIDs. Integrating transcriptomic data analysis, we found that the majority of upregulated differentially expressed genes were significantly enriched near specific CID boundaries. Specifically, we comprehensively elucidated that the nucleoid-associated protein DrEbfC as a global regulatory factor for gene expression, may modulate the efficiency of relevant metabolic pathways by altering the local chromosome structure, thereby influencing the physiological state of the bacterium. Overall, our study revealed the chromosome conformations of D. radiodurans under different conditions and offered valuable insights into the molecular response mechanism of this extremophile to survival stresses.