Promoted growth with dynamic cellular stoichiometry driven by utilization of in-situ dissolved organic matter: Insights from bloom-forming dinoflagellate Prorocentrum donghaiense.
Hongwei Wang, Siyang Wu, Jian Ma, Yiting Hong, Chentao Guo, Jing Zhao, Xin Lin
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引用次数: 0
Abstract
Mixotrophic dinoflagellates frequently cause harmful algal blooms (HABs) in eutrophic waters that contain diverse dissolved organic matter (DOM), especially intensive mariculture areas. Compared to the extensive investigation of phagotrophy and single organic molecule uptake by causative species, we have limited knowledge about the capability of mixotrophic dinoflagellates to utilize in-situ DOM in mariculture waters and its contribution to HABs. Here we use filtered in-situ mariculture water as the sole medium to examine the physiological response of Prorocentrum donghaiense to the natural mariculture DOM. Our results showed an 87.2% increase in the cell growth rate, as well as photosynthesis (16.8%-29.2%) and cellular chlorophyll a (32.4%-70.7%) when cultured with DOM compared to those grown in the inorganic medium. Meanwhile, cellular stoichiometry varied greatly among the groups supplied with mariculture DOM of different seasons, and the ecological implications were then discussed. Additionally, parallel cultures revealed the phycosphere bacterioplankton community compete with the algae cell regarding the nutrient utilization. This study quantifies the efficient utilization of in-situ mariculture DOM by P. donghaiense and indicates its vital role in sustaining HAB events and great effects on the biogeochemical cycle.
在含有多种溶解有机物（DOM）的富营养化水域，特别是密集的海水养殖区，混养甲藻经常会引起有害藻华（HABs）。与对致病物种的吞噬能力和单一有机分子吸收能力的广泛研究相比，我们对混养甲藻利用海产养殖水域原位 DOM 的能力及其对 HABs 的贡献了解有限。在此，我们使用过滤后的原位海水养殖水作为唯一介质，研究了 Prorocentrum donghaiense 对天然海水养殖 DOM 的生理反应。结果表明，与在无机培养基中生长的细胞相比，用 DOM 培养的细胞生长速度、光合作用（16.8%-29.2%）和细胞叶绿素 a（32.4%-70.7%）均提高了 87.2%。同时，使用不同季节的海水养殖 DOM 培养的各组之间的细胞化学计量学差异很大，并对其生态学意义进行了讨论。此外，平行培养揭示了藻层细菌群落与藻细胞在营养物质利用方面的竞争。这项研究量化了东海栉孔扇贝对原位海产养殖 DOM 的有效利用，表明其在维持 HAB 事件中的重要作用以及对生物地球化学循环的巨大影响。
期刊介绍：
Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes.
Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following:
– The extent, persistence, and consequences of change and the recovery from such change in natural marine systems
– The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems
– The biogeochemistry of naturally occurring and anthropogenic substances
– Models that describe and predict the above processes
– Monitoring studies, to the extent that their results provide new information on functional processes
– Methodological papers describing improved quantitative techniques for the marine sciences.
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