Band Visibility in High-Resolution Optical Coherence Tomography Assessed With a Custom Review Tool and Updated, Histology-Derived Nomenclature.

IF 2.6 3区医学 Q2 OPHTHALMOLOGY Translational Vision Science & Technology Pub Date : 2024-12-02 DOI:10.1167/tvst.13.12.19

Lukas Goerdt, Thomas A Swain, Deepayan Kar, Gerald McGwin, Andreas Berlin, Mark E Clark, Cynthia Owsley, Kenneth R Sloan, Christine A Curcio

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Abstract

Purpose: For structure-function research at the transition of aging to age-related macular degeneration, we refined the current consensus optical coherence tomography (OCT) nomenclature and evaluated a novel review software for investigational high-resolution OCT imaging (HR-OCT; <3 µm axial resolution).

Method: Volume electron microscopy, immunolocalizations, histology, and investigational devices informed a refined OCT nomenclature for a custom ImageJ-based review tool to assess retinal band visibility. We examined effects on retinal band visibility of automated real-time averaging (ART) 9 and 100 (11 eyes of 10 healthy young adults), aging (10 young vs 22 healthy aged), and age-related macular degeneration (AMD; 22 healthy aged, 17 early (e)AMD, 15 intermediate (i)AMD). Intrareader reliability was assessed.

Results: Bands not included in consensus nomenclature are now visible using HR-OCT: inner plexiform layer (IPL) 1-5, outer plexiform layer (OPL) 1-2, outer segment interdigitation zone 1-2 (OSIZ, including hyporeflective outer segments), and retinal pigment epithelium (RPE) 1-5. Cohen's kappa was 0.54-0.88 for inner and 0.67-0.83 for outer retinal bands in a subset of 10 eyes. IPL-3-5 and OPL-2 visibility benefitted from increased ART. OSIZ-2 and RPE-1,2,3,5 visibility was worse in aged eyes than in young eyes. OSIZ-1-2, RPE-1, and RPE-5 visibility decreased in eAMD and iAMD compared to healthy aged eyes.

Conclusions: We reliably identified 28 retinal bands using a novel review tool for HR-OCT. Image averaging improved inner retinal band visibility. Aging and AMD development impacted outer retinal band visibility.

Translational significance: Detailed knowledge of anatomic structures visible on OCT will enhance precision in research, including AI training and structure-function analyses.

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目的：为了对老龄化向老年性黄斑变性过渡阶段的结构-功能进行研究，我们改进了目前一致认可的光学相干断层扫描（OCT）术语，并评估了一种新型审查软件，用于研究性高分辨率 OCT 成像（HR-OCT；方法：通过电子显微镜、免疫定位、组织学和研究性设备，为基于 ImageJ 的定制审查工具评估视网膜带能见度提供了改进的 OCT 术语：体积电子显微镜、免疫定位、组织学和研究设备为基于 ImageJ 的定制审查工具提供了改进的 OCT 术语，以评估视网膜带能见度。我们研究了自动实时平均（ART）9 和 100（10 位健康年轻人的 11 只眼睛）、老龄化（10 位年轻人与 22 位健康老年人）和年龄相关性黄斑变性（AMD；22 位健康老年人、17 位早期（e）AMD、15 位中期（i）AMD）对视网膜带能见度的影响。对读数器内部的可靠性进行了评估：使用 HR-OCT 现在可以看到共识命名法中未包括的波段：内层丛状层 (IPL) 1-5、外层丛状层 (OPL) 1-2、外节段交错区 1-2（OSIZ，包括低反射外节段）和视网膜色素上皮 (RPE) 1-5。在 10 只眼睛的子集中，视网膜内层带的 Cohen's kappa 为 0.54-0.88，视网膜外层带的 Cohen's kappa 为 0.67-0.83。IPL-3-5 和 OPL-2 的能见度得益于 ART 的增加。老年眼的 OSIZ-2 和 RPE-1,2,3,5 能见度比年轻眼差。与健康的老年眼相比，eAMD 和 iAMD 患者的 OSIZ-1-2、RPE-1 和 RPE-5 可见度降低：我们使用 HR-OCT 的新型审查工具可靠地识别了 28 个视网膜带。图像平均化提高了内层视网膜带的可见度。老化和 AMD 的发展影响了外层视网膜带的可见度：详细了解 OCT 上可见的解剖结构将提高研究的精确度，包括人工智能训练和结构功能分析。

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来源期刊

Translational Vision Science & Technology Engineering-Biomedical Engineering

CiteScore

5.70

自引率

3.30%

发文量

346

审稿时长

25 weeks

期刊介绍： Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.