A long noncoding eRNA forms R-loops to shape emotional experience-induced behavioral adaptation.

IF 44.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Science Pub Date : 2024-12-13 Epub Date: 2024-12-12 DOI:10.1126/science.adp1562
Rose Marie Akiki, Rebecca G Cornbrooks, Kosuke Magami, Alain Greige, Kirsten K Snyder, Daniel J Wood, Mary Claire Herrington, Philip Mace, Kyle Blidy, Nobuya Koike, Stefano Berto, Christopher W Cowan, Makoto Taniguchi
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Abstract

Emotional experiences often evoke neural plasticity that supports adaptive changes in behavior, including maladaptive plasticity associated with mood and substance use disorders. These adaptations are supported in part by experience-dependent activation of immediate-early response genes, such as Npas4 (neuronal PAS domain protein 4). Here we show that a conserved long noncoding enhancer RNA (lnc-eRNA), transcribed from an activity-sensitive enhancer, produces DNA:RNA hybrid R-loop structures that support three-dimensional chromatin looping between enhancer and proximal promoter and rapid Npas4 gene induction. Furthermore, in mouse models, Npas4 lnc-eRNA and its R-loop are required for the development of behavioral adaptations produced by chronic psychosocial stress or cocaine exposure, revealing a potential role for this regulatory mechanism in the transmission of emotional experiences.

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情绪体验通常会唤起神经可塑性,从而支持行为的适应性变化,包括与情绪障碍和药物使用障碍相关的不适应可塑性。这些适应性在一定程度上是由经验依赖性激活的即时早期反应基因(如 Npas4(神经元 PAS 结构域蛋白 4))支持的。在这里,我们发现了一种保守的长非编码增强子 RNA(lnc-eRNA),这种 RNA 从活性敏感的增强子转录而来,产生了 DNA:RNA 混合 R 循环结构,支持增强子和近端启动子之间的三维染色质循环以及 Npas4 基因的快速诱导。此外,在小鼠模型中,Npas4 lnc-eRNA 及其 R 环是慢性社会心理压力或可卡因暴露所产生的行为适应性发展所必需的,揭示了这种调控机制在情感体验传递中的潜在作用。
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来源期刊
Science
Science 综合性期刊-综合性期刊
CiteScore
61.10
自引率
0.90%
发文量
0
审稿时长
2.1 months
期刊介绍: Science is a leading outlet for scientific news, commentary, and cutting-edge research. Through its print and online incarnations, Science reaches an estimated worldwide readership of more than one million. Science’s authorship is global too, and its articles consistently rank among the world's most cited research. Science serves as a forum for discussion of important issues related to the advancement of science by publishing material on which a consensus has been reached as well as including the presentation of minority or conflicting points of view. Accordingly, all articles published in Science—including editorials, news and comment, and book reviews—are signed and reflect the individual views of the authors and not official points of view adopted by AAAS or the institutions with which the authors are affiliated. Science seeks to publish those papers that are most influential in their fields or across fields and that will significantly advance scientific understanding. Selected papers should present novel and broadly important data, syntheses, or concepts. They should merit recognition by the wider scientific community and general public provided by publication in Science, beyond that provided by specialty journals. Science welcomes submissions from all fields of science and from any source. The editors are committed to the prompt evaluation and publication of submitted papers while upholding high standards that support reproducibility of published research. Science is published weekly; selected papers are published online ahead of print.
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