A novel scaffold for biofilm formation by soil microbes using iron-cross-linked alginate gels.

Abstract

This study aimed to evaluate the suitability of alginate gels, specifically ferric-ion-cross-linked alginate (Fe-alginate) and calcium-ion-cross-linked alginate (Ca-alginate), as scaffolds for soil microbial attachment and biofilm formation in soil. Staining with crystal violet and observations with scanning electron microscopy showed that microorganisms formed biofilms on Fe-alginate surfaces in the soil. When the soil was incubated with Fe-alginate, microbial biomass, estimated by adenosine triphosphate content, increased not only in the Fe-alginate but also in the surrounding soil. The weight of Ca-alginate in the soil decreased with time owing to chemical dissolution. However, the weight of Fe-alginate in the soil did not decrease, likely because it was protected by the microbial biofilm that formed on its surface. These results demonstrate that the use of Fe-alginate, in contrast to Ca-alginate, as a scaffold may allow for more efficient use of soil microbial functions in agriculture and bioremediation.