Mesenchymal stem cell-derived exosomes carry miR-125a-5p to improve diabetic keratopathy by regulating endoplasmic reticulum stress.

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2024-12-05 DOI:10.1016/j.tice.2024.102669

Weina Li, Shiping He, Chaoqun Lin, Sheng Yang, Wenbin Zhang

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Abstract

Background: Diabetic keratopathy is a prevalent but sometimes ignored visual condition in diabetic patients, which significantly affects patients with diabetes mellitus (DM) in terms of their visual acuity. Exosomes regulate diabetes-related conditions like diabetic keratopathy (DK) by secreting their components into the body.

Objective: Aim to investigate the effect and mechanism of mesenchymal stem cell (MSC)-derived exosome miR-125a-5p on DK.

Methods: Transmission electron microscopy, along with nanoparticle tracking analysis, was used to determine the morphology and size of exosomes. To evaluate cell viability, proliferation, and migration, Western blotting and RT-qPCR methods were used. CCK-8, cell cloning, and scratch assays were used to measure protein levels and mRNA expression.

Results: High glucose treatment of corneal epithelial cells weakened cell viability, proliferation and migration, and the level of miR-125a-5p was significantly reduced. It has been proposed that elevated levels of miR-125a-5p could enhance cell viability, proliferation, and migration, can inhibit endoplasmic reticulum stress induced by high glucose, which is the same as the effect of endoplasmic reticulum stress inhibitors.

Conclusion: Mouse bone marrow MSC-derived exosome miR-125a-5p repairs corneal epithelial cell viability and proliferation as well as migration ability to improve DK by inhibiting high glucose-induced endoplasmic reticulum stress.

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间充质干细胞衍生的外泌体携带 miR-125a-5p，通过调节内质网应激改善糖尿病角膜病变。

背景：糖尿病角膜病变是糖尿病患者普遍存在但有时被忽视的视力问题，它严重影响糖尿病（DM）患者的视力。外泌体通过向体内分泌其成分来调节糖尿病相关病症，如糖尿病角膜病（DK）：旨在研究间充质干细胞（MSC）衍生的外泌体 miR-125a-5p 对 DK 的影响和机制：方法：利用透射电子显微镜和纳米粒子跟踪分析确定外泌体的形态和大小。为了评估细胞活力、增殖和迁移，采用了 Western 印迹和 RT-qPCR 方法。CCK-8、细胞克隆和划痕试验用于测量蛋白质水平和 mRNA 表达：结果：角膜上皮细胞经高糖处理后，细胞活力、增殖和迁移能力减弱，miR-125a-5p 水平显著降低。有研究认为，miR-125a-5p水平升高能增强细胞活力、增殖和迁移，能抑制高糖诱导的内质网应激，这与内质网应激抑制剂的作用相同：结论：小鼠骨髓间充质干细胞衍生的外泌体miR-125a-5p通过抑制高糖诱导的内质网应激，修复角膜上皮细胞的活力、增殖和迁移能力，从而改善DK。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.