Determination of Limits for Evaluating the Degree of Internalization of γ-Fe2O3 Nanoparticles by Cultures of Human Mesenchymal Stomal Cells

IF 1.4 4区化学 Q4 CHEMISTRY, PHYSICAL Colloid Journal Pub Date : 2024-12-17 DOI:10.1134/S1061933X24600738

E. A. Burban, F. A. Fadeyev, A. P. Safronov, F. A. Blyakhman, T. V. Terzian, D. S. Neznakhin, A. A. Yushkov, G. V. Kurlyandskaya

{"title":"Determination of Limits for Evaluating the Degree of Internalization of γ-Fe2O3 Nanoparticles by Cultures of Human Mesenchymal Stomal Cells","authors":"E. A. Burban, F. A. Fadeyev, A. P. Safronov, F. A. Blyakhman, T. V. Terzian, D. S. Neznakhin, A. A. Yushkov, G. V. Kurlyandskaya","doi":"10.1134/S1061933X24600738","DOIUrl":null,"url":null,"abstract":"A culture of human bone marrow mesenchymal stromal cells (MSCs) has been investigated. The cell culture has been grown as a monolayer in a nutrient medium containing a stabilized aqueous suspension of magnetic maghemite (γ-Fe2O3) nanoparticles (MNPs). The MNPs have been synthesized by the electrophysical method of laser target evaporation. A method has been proposed for stabilizing the suspension in the nutrient medium with a high ionic strength. The possibility of MNP internalization (either by fixing on the cell membrane or by incorporating into the cell space) with human MSCs has been evaluated using optical microscopy, scanning (SEM) and transmission (TEM) electron microscopy, and SQUID magnetometry. Comparative analysis of the structure and magnetic properties has been performed, and assumptions have been put forward about the features of MNP internalization with the cells in this system. It has been revealed that the limiting amount of MNPs that can be reliably analyzed in a biological sample of the studied type is about 0.005 mg. It has been found that, in the considered range of initial concentrations of magnetic nanoparticles in biological samples based on human MSCs, the level of accumulation of magnetic nanoparticles in cell cultures depends on their concentration.","PeriodicalId":521,"journal":{"name":"Colloid Journal","volume":"86 6","pages":"836 - 847"},"PeriodicalIF":1.4000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloid Journal","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1134/S1061933X24600738","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

A culture of human bone marrow mesenchymal stromal cells (MSCs) has been investigated. The cell culture has been grown as a monolayer in a nutrient medium containing a stabilized aqueous suspension of magnetic maghemite (γ-Fe₂O₃) nanoparticles (MNPs). The MNPs have been synthesized by the electrophysical method of laser target evaporation. A method has been proposed for stabilizing the suspension in the nutrient medium with a high ionic strength. The possibility of MNP internalization (either by fixing on the cell membrane or by incorporating into the cell space) with human MSCs has been evaluated using optical microscopy, scanning (SEM) and transmission (TEM) electron microscopy, and SQUID magnetometry. Comparative analysis of the structure and magnetic properties has been performed, and assumptions have been put forward about the features of MNP internalization with the cells in this system. It has been revealed that the limiting amount of MNPs that can be reliably analyzed in a biological sample of the studied type is about 0.005 mg. It has been found that, in the considered range of initial concentrations of magnetic nanoparticles in biological samples based on human MSCs, the level of accumulation of magnetic nanoparticles in cell cultures depends on their concentration.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

人间充质造口细胞培养评价γ-Fe2O3纳米颗粒内化程度极限值的确定

研究了人骨髓间充质基质细胞（MSCs）的培养。细胞培养物在含有磁性磁赤铁矿（γ-Fe2O3）纳米颗粒（MNPs）的稳定水悬浮液的营养培养基中作为单层生长。采用激光靶蒸发的电物理方法合成了MNPs。提出了一种在高离子强度的营养介质中稳定悬浮液的方法。利用光学显微镜、扫描电镜（SEM）和透射电镜（TEM）以及SQUID磁强计，评估了MNP内化（通过固定在细胞膜上或通过并入细胞空间）与人间充质干细胞的可能性。对MNP的结构和磁性进行了比较分析，并对MNP内化的特点与该体系中的细胞进行了假设。研究表明，在所研究类型的生物样品中可以可靠分析的MNPs的极限量约为0.005 mg。研究发现，在基于人间充质干细胞的生物样品中磁性纳米颗粒的初始浓度范围内，细胞培养中磁性纳米颗粒的积累水平取决于它们的浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Colloid Journal 化学-物理化学

CiteScore

2.20

自引率

18.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Colloid Journal (Kolloidnyi Zhurnal) is the only journal in Russia that publishes the results of research in the area of chemical science dealing with the disperse state of matter and surface phenomena in disperse systems. The journal covers experimental and theoretical works on a great variety of colloid and surface phenomena: the structure and properties of interfaces; adsorption phenomena and structure of adsorption layers of surfactants; capillary phenomena; wetting films; wetting and spreading; and detergency. The formation of colloid systems, their molecular-kinetic and optical properties, surface forces, interaction of colloidal particles, stabilization, and criteria of stability loss of different disperse systems (lyosols and aerosols, suspensions, emulsions, foams, and micellar systems) are also topics of the journal. Colloid Journal also includes the phenomena of electro- and diffusiophoresis, electro- and thermoosmosis, and capillary and reverse osmosis, i.e., phenomena dealing with the existence of diffusion layers of molecules and ions in the vicinity of the interface.