{"title":"Metagenomics and metatranscriptomics insights into microbial enhancement of H<sub>2</sub>S removal and CO<sub>2</sub> assimilation.","authors":"Junjie Wang, Zhuowei Cheng, Yunfei Su, Jiade Wang, Dongzhi Chen, Jianmeng Chen, Xiaoming Wu, Aobo Chen, Zhenyu Gu","doi":"10.1016/j.jenvman.2024.123714","DOIUrl":null,"url":null,"abstract":"<p><p>This study focuses on the coupled process of bio-enhanced absorption and biodesulfurization for the toxic gas H<sub>2</sub>S and the greenhouse gas CO<sub>2</sub>. The results show that on the basis of stabilized absorption of H<sub>2</sub>S and CO<sub>2</sub> by alkaline solution (Stage I), the addition of air-lift bioreactor process solution in the absorption column enhanced their absorption (Stage II). Specifically, at constant inlet concentrations of H₂S and CO₂ of 3% (30,000 ppmv) and 30% (300,000 ppmv), respectively, the outlet gases were primarily H₂S, CO₂, and N₂. And the outlet H<sub>2</sub>S and CO<sub>2</sub> concentrations decreased from 10,038 ± 1166 ppmv and 49,897 ± 2545 ppmv in Stage I to 940 ± 163 ppmv and 21,000 ± 2165 ppmv in Stage II. S<sup>0</sup>-producing performance (348 ± 20-503 ± 23 mg S/L) and biomass concentration (467 ± 13-677 ± 55 mg/L) in the subsequent bioreactor also increased in response to the enhanced absorption of H<sub>2</sub>S and CO<sub>2</sub>. Biologically enhanced H<sub>2</sub>S and CO<sub>2</sub> absorption differs from physicochemical factors in that it depends on several physiological parameters such as microbial community composition and gene expression levels. In this study, the sulfur autotrophic denitrifying bacteria Thioalkalivibrio and Arenimonas had high abundance and activity (abundance: 69.5% and 21.1%, expression: 82.4% and 13.9%), and they were the main contributors to the bio-enhanced absorption of H<sub>2</sub>S and CO<sub>2</sub> in this system. In addition, the main factor for enhanced H<sub>2</sub>S absorption could be the high expression of sulfide:quinone oxidoreductase (SQR, encoding gene sqr) (45 ± 9 to 821 ± 102 transcripts per million). Enhanced CO<sub>2</sub> absorption could have been achieved by the oxidation of more H<sub>2</sub>S generating more energy to increase the carboxylation activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, encoding genes rbcLS). Enhanced H<sub>2</sub>S absorption enhances CO<sub>2</sub> absorption and facilitates microbial growth, which in turn benefits the metabolism of H<sub>2</sub>S, creating a complementary biologically enhanced absorption. This study provides a novel strategy, demonstrating the potential of autotrophic sulfide-oxidizing microorganisms in the simultaneous removal of H₂S and assimilation of CO₂, and offers a deeper understanding of the underlying mechanisms.</p>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"373 ","pages":"123714"},"PeriodicalIF":8.0000,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Environmental Management","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jenvman.2024.123714","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
This study focuses on the coupled process of bio-enhanced absorption and biodesulfurization for the toxic gas H2S and the greenhouse gas CO2. The results show that on the basis of stabilized absorption of H2S and CO2 by alkaline solution (Stage I), the addition of air-lift bioreactor process solution in the absorption column enhanced their absorption (Stage II). Specifically, at constant inlet concentrations of H₂S and CO₂ of 3% (30,000 ppmv) and 30% (300,000 ppmv), respectively, the outlet gases were primarily H₂S, CO₂, and N₂. And the outlet H2S and CO2 concentrations decreased from 10,038 ± 1166 ppmv and 49,897 ± 2545 ppmv in Stage I to 940 ± 163 ppmv and 21,000 ± 2165 ppmv in Stage II. S0-producing performance (348 ± 20-503 ± 23 mg S/L) and biomass concentration (467 ± 13-677 ± 55 mg/L) in the subsequent bioreactor also increased in response to the enhanced absorption of H2S and CO2. Biologically enhanced H2S and CO2 absorption differs from physicochemical factors in that it depends on several physiological parameters such as microbial community composition and gene expression levels. In this study, the sulfur autotrophic denitrifying bacteria Thioalkalivibrio and Arenimonas had high abundance and activity (abundance: 69.5% and 21.1%, expression: 82.4% and 13.9%), and they were the main contributors to the bio-enhanced absorption of H2S and CO2 in this system. In addition, the main factor for enhanced H2S absorption could be the high expression of sulfide:quinone oxidoreductase (SQR, encoding gene sqr) (45 ± 9 to 821 ± 102 transcripts per million). Enhanced CO2 absorption could have been achieved by the oxidation of more H2S generating more energy to increase the carboxylation activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, encoding genes rbcLS). Enhanced H2S absorption enhances CO2 absorption and facilitates microbial growth, which in turn benefits the metabolism of H2S, creating a complementary biologically enhanced absorption. This study provides a novel strategy, demonstrating the potential of autotrophic sulfide-oxidizing microorganisms in the simultaneous removal of H₂S and assimilation of CO₂, and offers a deeper understanding of the underlying mechanisms.
期刊介绍:
The Journal of Environmental Management is a journal for the publication of peer reviewed, original research for all aspects of management and the managed use of the environment, both natural and man-made.Critical review articles are also welcome; submission of these is strongly encouraged.