CFI-1 functions unilaterally to restrict gap junction formation in C. elegans.

IF 3.6 2区生物学 Q1 DEVELOPMENTAL BIOLOGY Development Pub Date : 2025-01-01 Epub Date: 2025-01-07 DOI:10.1242/dev.202955

Zan Wu, Lin Pang, Mei Ding

引用次数: 0

Abstract

Electrical coupling is vital to neural communication, facilitating synchronized activity among neurons. Despite its significance, the precise mechanisms governing the establishment of gap junction connections between specific neurons remain elusive. Here, we identified that the PVC interneuron in Caenorhabditis elegans forms gap junction connections with the PVR interneuron. The transcriptional regulator CFI-1 (ARID3) is specifically expressed in the PVC but not PVR interneuron. Reducing cfi-1 expression in the PVC interneuron leads to enhanced gap junction formation in the PVR neuron, while ectopic expression of cfi-1 in the PVR neuron restores the proper level of gap junction connections in the PVC neuron, along with the normal touch response. These findings unveil the pivotal role of CFI-1 in bidirectionally regulating the formation of gap junctions within a specific neuronal pair, shedding light on the intricate molecular mechanisms governing neuronal connectivity in vivo.

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CFI-1/ARID3具有单侧功能，可限制优雅小鼠间隙连接的形成。

电耦合对神经通讯至关重要，促进神经元之间的同步活动。尽管它具有重要意义，但控制特定神经元之间建立间隙连接的精确机制仍然难以捉摸。在这里，我们发现秀丽隐杆线虫的PVC中间神经元与PVR中间神经元形成间隙连接连接。转录调节因子CFI-1/ARID3在PVC而非PVR中间神经元中特异性表达。降低PVC中间神经元中cfi-1的表达可导致PVR神经元中间隙连接形成增强，而PVR神经元中cfi-1的异位表达可恢复PVC神经元中适当水平的间隙连接连接，同时恢复正常的触摸反应。这些发现揭示了CFI-1/ARID3在双向调节特定神经元对间隙连接形成中的关键作用，揭示了体内控制神经元连接的复杂分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

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