Specific growth rates and growth stoichiometries of Saccharomycotina yeasts on ethanol as sole carbon and energy substrate.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY FEMS yeast research Pub Date : 2024-01-09 DOI:10.1093/femsyr/foae037
Marieke Warmerdam, Marcel A Vieira-Lara, Robert Mans, Jean Marc Daran, Jack T Pronk
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Abstract

Emerging low-emission production technologies make ethanol an interesting substrate for yeast biotechnology, but information on growth rates and biomass yields of yeasts on ethanol is scarce. Strains of 52 Saccharomycotina yeasts were screened for growth on ethanol. The 21 fastest strains, among which representatives of the Phaffomycetales order were overrepresented, showed specific growth rates in ethanol-grown shake-flask cultures between 0.12 and 0.46 h-1. Seven strains were studied in aerobic, ethanol-limited chemostats (dilution rate 0.10 h-1). Saccharomyces cerevisiae and Kluyveromyces lactis, whose genomes do not encode Complex-I-type NADH dehydrogenases, showed biomass yields of 0.59 and 0.56 gbiomass gethanol-1, respectively. Different biomass yields were observed among species whose genomes do harbour Complex-I-encoding genes: Phaffomyces thermotolerans (0.58 g g-1), Pichia ethanolica (0.59 g g-1), Saturnispora dispora (0.66 g g-1), Ogataea parapolymorpha (0.67 g g-1), and Cyberlindnera jadinii (0.73 g g-1). Cyberlindnera jadinii biomass showed the highest protein content (59 ± 2%) of these yeasts. Its biomass yield corresponded to 88% of the theoretical maximum that is reached when growth is limited by assimilation rather than by energy availability. This study suggests that energy coupling of mitochondrial respiration and its regulation will become key factors for selecting and improving yeast strains for ethanol-based processes.

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新兴的低排放生产技术使乙醇成为酵母生物技术感兴趣的底物,但有关酵母在乙醇上的生长率和生物量产量的信息却很少。研究人员筛选了 52 株酵母菌在乙醇上的生长情况。在乙醇培养的摇瓶培养物中,21 个生长速度最快的菌株(其中法氏酵母菌纲的代表菌株较多)的特定生长率在 0.12 至 0.46 h-1 之间。在有氧、乙醇限制的恒温器(稀释率为 0.10 h-1)中研究了 7 个菌株。其基因组未编码复合物 I 型 NADH 脱氢酶的酿酒酵母(Saccharomyces cerevisiae)和乳酸克鲁维酵母(Kluyveromyces lactis)的生物质产量分别为 0.59 和 0.56 gbiomass∙gethanol-1 。在基因组中含有复合酶 I 编码基因的物种中,生物量产量也有所不同:这些物种包括:热耐菌 Phaffomyces thermotolerans(0.58 g∙g-1)、Pichia ethanolica(0.59 g∙g-1)、Saturnispora dispora(0.66 g∙g-1)、Ogataea parapolymorpha(0.67 g∙g-1)和 Cyberlindnera jadinii(0.73 g∙g-1)。在这些酵母中,C. jadinii 的生物量显示出最高的蛋白质含量(59 ± 2%)。它的生物量产量相当于理论最大值的 88%,而理论最大值是由同化作用而不是能量供应限制生长时达到的。这项研究表明,线粒体呼吸的能量耦合及其调节将成为选择和改良乙醇法酵母菌株的关键因素。
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来源期刊
FEMS yeast research
FEMS yeast research 生物-生物工程与应用微生物
CiteScore
5.70
自引率
6.20%
发文量
54
审稿时长
1 months
期刊介绍: FEMS Yeast Research offers efficient publication of high-quality original Research Articles, Mini-reviews, Letters to the Editor, Perspectives and Commentaries that express current opinions. The journal will select for publication only those manuscripts deemed to be of major relevance to the field and generally will not consider articles that are largely descriptive without insights on underlying mechanism or biology. Submissions on any yeast species are welcome provided they report results within the scope outlined below and are of significance to the yeast field.
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