Increased lamina propria B cells play roles in fructose-induced hypertension of Dahl salt-sensitive rats.

IF 5.2 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Life sciences Pub Date : 2024-12-14 DOI:10.1016/j.lfs.2024.123314

Sungmin Jang, Cheong-Wun Kim, Zainab Yetunde Olarinoye, Sadia Akter, Inkyeom Kim

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Abstract

Aims: Although the immune system participates in the development of hypertension, the proportional contributions of distinct immune cells remain poorly understood. With the development of transcriptomics, we can profile the transcriptomes of individual immune cells and assess the relative contribution of each immune cell to the development of hypertension. So, we tested the hypothesis that increased lamina propria B cells play roles in fructose-induced hypertension of Dahl salt-sensitive (SS) rats.

Materials and methods: Eight-week-old Dahl SS and Dahl salt-resistant (SR) male rats were divided into four groups; each group received either tap water (TW) or a 20 % fructose solution (HFS) for 4 weeks. Systolic blood pressure was measured using the tail-cuff method. Single-cell RNA sequencing (scRNA-seq) analysis was performed on lamina propria (LP) cells and peripheral blood mononuclear cells (PBMCs) obtained from the SS and SR rats subjected to either TW or HFS.

Key findings: Results revealed that high-fructose intake induced hypertension in the SS rats but not in the SR rats. It also increased B cells in LPs but not in PBMCs of the SS rats; their subsets showed increased follicular and naïve B cells. Increased lamina propria B cells play roles in fructose-induced hypertension of SS rats.

Significance: This finding suggest that targeting B cells could be a potential strategy to mitigate high blood pressure in fructose-induced hypertension.

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目的：尽管免疫系统参与了高血压的发病，但人们对不同免疫细胞的作用比例仍然知之甚少。随着转录组学的发展，我们可以分析单个免疫细胞的转录组，评估每个免疫细胞对高血压发生的相对贡献。因此，我们测试了固有层 B 细胞增加在果糖诱导的 Dahl 盐敏感（SS）大鼠高血压中发挥作用的假设：将8周大的Dahl SS和Dahl耐盐（SR）雄性大鼠分为4组，每组接受自来水（TW）或20%果糖溶液（HFS）4周。采用尾袖法测定收缩压。对从接受 TW 或 HFS 的 SS 和 SR 大鼠体内获得的固有层（LP）细胞和外周血单核细胞（PBMC）进行了单细胞 RNA 测序（scRNA-seq）分析：研究结果表明，高果糖摄入会诱发 SS 大鼠的高血压，但不会诱发 SR 大鼠的高血压。高果糖还增加了 SS 大鼠固有层 B 细胞的数量，但没有增加 SS 大鼠 PBMCs 中的 B 细胞数量。固有层 B 细胞的增加在果糖诱导的 SS 大鼠高血压中发挥作用：这一发现表明，针对 B 细胞可能是缓解果糖诱导的高血压的一种潜在策略。

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来源期刊

Life sciences 医学-药学

CiteScore

12.20

自引率

1.60%

发文量

841

审稿时长

6 months

期刊介绍： Life Sciences is an international journal publishing articles that emphasize the molecular, cellular, and functional basis of therapy. The journal emphasizes the understanding of mechanism that is relevant to all aspects of human disease and translation to patients. All articles are rigorously reviewed. The Journal favors publication of full-length papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research. The Journal welcomes reviews on topics of wide interest to investigators in the life sciences. We particularly encourage submission of brief, focused reviews containing high-quality artwork and require the use of mechanistic summary diagrams.