ClBRN1 from Chrysanthemum lavandulifolium enhances the stress resistance of transgenic Arabidopsis.

IF 2.3 3区 生物学 Q2 MULTIDISCIPLINARY SCIENCES PeerJ Pub Date : 2024-12-12 eCollection Date: 2024-01-01 DOI:10.7717/peerj.18620
Yanxi Li, Wenting He, Yueyue Liu, Chendi Mei, Hai Wang, Xuebin Song
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引用次数: 0

Abstract

Background: Chrysanthemum (Chrysanthemum×morifolium Ramat.) is a particularly important autumn perennial flower for potted plant, flower bed and border, and cut flower with high ornamental value. However, abiotic stress can affect the ornamental quality of Chrysanthemum. NAC (NAM, ATAF1-2, and CUC2) transcription factors (TFs) play an important role in regulating plant growth and development, as well as responding to abiotic stresses.

Methods: In this study, the ClBRN1 (Chrysanthemum lavandulifolium BEARSKIN gene) was isolated from the Chrysanthemum model plant C. lavandulifolium. And analyze the function of the gene through bioinformatics, subcellular localization and overexpression.

Results: Bioinformatics analysis showed that the ClBRN1 gene was a member of the NAC TFs family, with a CDS (coding sequence) length of 1,080 bp and encoding 359 amino acids. The subcellular localization results found that the gene was located in the nucleus and cell membrane. Furthermore, the transgenic results in Arabidopsis thaliana showed that the gene significantly reduces plant height while improving salt and low temperature tolerance. Observation of paraffin sections of Arabidopsis stems also revealed that the secondary cell wall of overexpressing Arabidopsis stems was significantly thicker than that of wild-type. The above results indicate that the ClBRN1 gene may play an important role in regulating plant resistance to abiotic stress. This study will provide new insights for molecular breeding of resistant chrysanthemums in the future.

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PeerJ
PeerJ MULTIDISCIPLINARY SCIENCES-
CiteScore
4.70
自引率
3.70%
发文量
1665
审稿时长
10 weeks
期刊介绍: PeerJ is an open access peer-reviewed scientific journal covering research in the biological and medical sciences. At PeerJ, authors take out a lifetime publication plan (for as little as $99) which allows them to publish articles in the journal for free, forever. PeerJ has 5 Nobel Prize Winners on the Board; they have won several industry and media awards; and they are widely recognized as being one of the most interesting recent developments in academic publishing.
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