PeerJ最新文献

Background: Fructose 1,6-bisphosphatase 1 (FBP1) has been considered as a potential prognostic biomarker in glioblastoma (GBM), and this study explored the underlying mechanism.

Methods: The expression and effect of FBP1 expression on the prognosis of GBM patients were examined applying bioinformatics analyses. After measuring the expression of FBP1 in normal glial cell line HEB and GBM cells, cell counting kit-8 (CCK-8), 5-ethynyl-2-deoxyuridine (EdU), colony formation, transwell, and wound healing assay were carried out to examine the effects of silencing FBP1 on the proliferation and invasion of GBM cells. Aerobic glycolysis was measured by calculating the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of FBP1-silenced GBM cells. Furthermore, the protein levels of the mediators related to PI3K/AKT pathway and BCL2 protein family were detected via immunoblotting. Additionally, the effects of FBP1 silencing on the macrophage M2 polarization were assessed based on the fluorescence intensity of CD206 and the phosphorylation of STAT6 quantified by immunofluorescence and immunoblotting, respectively.

Results: High-expressed FBP1 was indicative of a worse prognosis of GBM. FBP1 knockdown in GBM cells suppressed the proliferation, invasion, migration, and aerobic glycolysis of GBM cells, lowered the phosphorylation levels of AKT and PI3K and the protein expression of BCL2 but promoted BAX protein expression. Moreover, FBP1 knockdown reduced CD206 fluorescence intensity and the phosphorylation of STAT6.

Conclusion: To conclude, FBP1 could be considered as a biomarker that affected the malignant phenotypes and aerobic glycolysis in GBM, contributing to the diagnosis and treatment of GBM.

Background: Osteoporosis is a common condition affecting middle-aged and older people, posing a serious threat to their health-related quality of life (HRQOL). In recent years, multiple studies have investigated the impact of exercise interventions on HRQOL in middle-aged and older individuals with osteoporosis, but the conclusions have been inconsistent. The aim of this study was to determine the true significance of exercise interventions on HRQOL in middle-aged and older individuals with osteoporosis and to identify optimal exercise prescriptions.

Methods: Six databases were searched for RCTs on the impact of exercise interventions on HRQOL in middle-aged and older individuals with osteoporosis. The methodological quality of the study was evaluated with Cochrane risk assessment tool. The effect size pooling, heterogeneity testing, and publication bias were analyzed using Review Manager 5.4 software.

Result: A total of 14 RCTs involving 1,214 participants were included, published between 2007 and 2022. The pooled results demonstrated that exercise interventions significantly improved general HRQOL (SMD = 0.79, 95% CI [0.34-1.24], p = 0.0006). In terms of physical HRQOL, significant improvements were observed in bodily pain (SMD = 0.51, 95% CI [0.24-0.78], p= 0.0002), physical function (SMD = 0.56, 95% CI [0.21-0.91], p = 0.002), role physical (SMD = 0.39, 95% CI [0.14-0.64], p = 0.003), and general health (SMD = 0.68, 95% CI [0.25-1.11], p = 0.002). Regarding mental HRQOL, significant improvements were found in vitality (SMD = 0.58, 95% CI [0.15-1.01], p = 0.008), social function (SMD = 0.37, 95% CI [0.17-0.58], p = 0.0004), and mental health (SMD = 0.50, 95% CI [0.25-0.74], p < 0.0001). Subgroup analysis results indicated that resistance training (SMD = 1.01, 95% CI [0.50-1.52], p = 0.0001), intervention frequency of at least three times per week (SMD = 0.80, 95% CI [0.22-1.38], p = 0.007), and intervention duration of 13-24 weeks (SMD = 0.85, 95% CI [0.37-1.33], p = 0.0005) had large and significant effects on general HRQOL improvements.

Conclusion: Exercise interventions improved HRQOL in middle-aged and older individuals with osteoporosis. Resistance training has shown greater benefits than mixed exercises. The optimal frequency is at least three per week, yielding the greatest improvement. Exercise interventions lasting 13-24 weeks had the most pronounced effect compared to other durations.

Registration: PROSPERO (No. CRD42023438771).

Background: Cognitive decline following cancer treatment can vary from mild cognitive impairment to severe dementia. However, there is inconsistent evidence regarding the relationship between breast cancer survivors and their risk of developing dementia. This meta-analysis aims to consolidate observational studies to explore the bidirectional association between breast cancer and dementia risk.

Methods: We conducted a comprehensive search using medical subject headings (MeSH) and keywords across PubMed, Cochrane Library, and Embase databases to identify cohort, case-control, and cross-sectional studies examining the link between breast cancer and dementia risk. Statistical analysis was performed using Stata version 14.0, with a random effects model employed to account for heterogeneity. Publication bias was assessed using funnel plots and Egger's test.

Results: This meta-analysis included 13 studies with a total of 346,051 participants, up to June 20, 2024. Of these, seven studies investigated the risk of dementia among patients with breast cancer, revealing a lower risk [OR = 0.56, 95% CI [0.27-1.18], I² = 99.1%, P = 0.128]. Similarly, seven studies explored the risk of breast cancer in individuals with dementia, showing a lower risk as well [OR = 0.79, 95% CI [0.51-1.22], I² = 94.5%, P = 0.290].

Conclusion: Our findings indicate that breast cancer is less likely to lead to dementia and that dementia is similarly low associated with risk of breast cancer. These insights are crucial for clinicians in guiding the prevention and monitoring of neurodegenerative conditions in patients with breast cancer.

Background: Larval propagation and seeding of scleractinian corals for restoration is a rapidly expanding field, with demonstrated applications to assist the recovery of declining populations on reefs. The process typically involves collecting coral reproductive material, facilitating in vitro fertilization (IVF), and settling and outplanting the resulting coral offspring. Optimizing IVF can reduce gamete wastage and increase larval yields for propagation, therefore improving the efficiency of this intervention.

Methods: In this study we tested three IVF conditions in four Caribbean broadcast-spawning coral species (i.e., Diploria labyrinthiformis, Colpophyllia natans, Pseudodiploria strigosa, Orbicella faveolata) to determine sperm concentration, gamete age, and co-incubation time resulting in the highest fertilization success. For each species, we exposed eggs from a single dam to pooled sperm samples from three sires (1) at concentrations ranging from zero to 10⁹ cell mL^-1, (2) after letting gametes age for 2 to 6 h, and (3) for a period of 15 to 120 min.

Results: These experiments revealed a gamete longevity of at least 4 h and clear minimum sperm concentration thresholds (>10⁵ to 10⁶ cell mL^-1) in all four species. Fertilization took place much faster than expected (≤15 min) in the three brain corals under study, whereas O. faveolata gametes required a co-incubation period of 60 to 120 min to achieve maximum IVF success.

Discussion: We present these results in the context of IVF data available for other hermaphroditic broadcast-spawning scleractinians. We then provide recommendations for coral breeding practitioners to maximize larval production from gamete collections, and finally, we discuss our findings' potential implications on fertilization dynamics during natural coral spawning events.

The present study examined the influence of gender stereotype information on cognitive judgments and impression evaluations of faces and voices. A 2 × 2 × 2 design was employed, with Perceptual Target (Face vs. Voice), Gender Stereotype Information (Consistent vs. Inconsistent) and Gender of Perceptual Targets (Male and Female) serving as within-subject factors. The results demonstrated that when gender stereotype information was consistent with the perceptual target's gender, response times for face gender judgments were shorter than for voice gender judgments. Nevertheless, the accuracy of gender judgments was higher for voices than faces. Furthermore, likability ratings for targets were significantly higher when gender stereotype information was consistent with the target than when it was inconsistent, for both face and voice judgments. These findings indicate that visual and auditory cues are processed differently in the context of gender judgments, thereby highlighting the distinct roles of facial and vocal information in gender perception. The current study contributes to understanding the complex interplay between gender stereotypes and multimodal social information processing.

Background: Soil microorganisms are crucial for plant growth, and both plants and their associated rhizosphere microbes are impacted by changes in soil moisture. Inoculation with beneficial fungi can improve bacterial community structure and soil parameters.

Aim: Under drought stress conditions, the effects of inoculation with Morchella on the physicochemical properties, enzyme activity, and bacterial community structure of the rhizosphere soil of Poa pratensis were studied.

Methods: High-throughput sequencing was employed to study rhizosphere soil bacterial communities in both Morchella-inoculated and uninoculated Poa pratensis rhizosphere soil subjected to moderate (50% soil moisture) and severe (30% soil moisture) drought stress, as well as under normal water conditions (70% soil moisture).

Results: Morchella inoculation significantly increased the alkaline nitrogen (AN) and available phosphorus (AP) contents, protease activity (PA), and alkaline phosphatase activity (APA) of Poa pratensis rhizosphere soil. Both Morchella inoculation and drought stress significantly altered the abundance and diversity of the P. pratensis rhizosphere community. The Chao1, Shannon, and Pielou diversity indices decreased with increasing drought stress. The effect of Morchella inoculation was improved under moderate drought stress and unstressed conditions. In addition, Morchella inoculation may help to stabilize the rhizosphere bacterial community under various levels of soil moisture.

Background: There is evidence that fine particulate matter (PM_2.5) exposure is associated with premature rupture of membranes (PROM); however, studies of its effect on term PROM (TPROM) are limited, and the results are inconsistent.

Objective: This study aimed to investigate the association between exposure to PM_2.5 and its components and the risk of TPROM.

Methods: From 2018 to 2022, we collected delivery data from pregnant women in Guangzhou. Using 1:1 case matching, we included 1,216 TPROM cases and 1,216 controls. PM_2.5 and its component concentrations were obtained from Tracking Air Pollution in China. The time-varying mean concentration method was used to estimate exposure to PM_2.5 and its components during different trimesters. Cox proportional hazards models were used to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) to evaluate the associations of exposure to PM_2.5 and its components with the risk of TPROM.

Results: The incidence of TPROM in this study was 19.66%. After adjusting for potential confounders, statistically significant associations were found between TPROM and exposure to PM_2.5, nitrate ( ${\mathrm{NO}}_3^{-}$ ), ammonium ( ${\mathrm{NH}}_4^{+}$ ), and black carbon (BC) during the second trimester and between TPROM and exposure to PM_2.5, sulphate ( ${\mathrm{SO}}_4^{2-}$ ), and BC during the third trimester. Specifically, the interquartile range (IQR) 3 (IQR3) and IQR4 of ${\mathrm{SO}}_4^{2-}$ exposure during the third trimester increased the risk of TPROM by 18% (95% CIs [1.01-1.39]) and 18% (95% CIs [1.01-1.39]), respectively. A nonlinear relationship was observed between exposure to PM_2.5, ${\mathrm{SO}}_4^{2-}$ , ${\mathrm{NH}}_4^{+}$ , and OM during the second trimester and the risk of TPROM. No significant interactions were found between PM_2.5 and its components with TPROM across various subgroups.

Conclusion: Our findings indicate significant associations between the risk of TPROM and exposure to PM_2.5 and several of its components during pregnancy. Contribute to the literature on the associations of PM_2.5 and its components with TPROM.

The primary effector cells involved in the formation of hypertrophic scars are fibroblasts. A potential peptide, ADSCP2 (adipose-derived stem cell peptide 2, the peptide fragment of ALCAM protein), derived from adipose-derived stem cell-conditioned medium, has been identified as having the potential to mitigate hypertrophic scar formation by targeting pyruvate carboxylase. However, the underlying mechanisms remain incompletely understood. Whether ADSCP2 attenuates hypertrophic scar fibrosis at the transcription level remains unclear. Consequently, this study sought to elucidate the potential mechanism associated with ADSCP2 by examining genome-wide transcriptional alterations and changes in chromatin accessibility in fibroblasts. This was achieved through the integrated analysis of assay for transposase accessible chromatin using sequencing (ATAC-seq) and RNA sequencing (RNA-seq). In the ADSCP2 treatment group, ATAC-seq identified a total of 7,805 differential peaks associated with 3,176 genes. RNA-seq analysis revealed 345 upregulated and 399 downregulated transcripts in the same group. A combined Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of both downregulated genes and close-ACRs (accessible chromatin regions) genes within the ADSCP2 treatment group indicated regulation of the oxidative phosphorylation pathway (OXPHOS) by ADSCP2. The amalgamation of ATAC-seq and RNA-seq data elucidates that two OXPHOS associated genes, namely COX6B1 (cytochrome c oxidase subunit 6B1) and NDUFA1 (NADH dehydrogenase (ubiquinone) alpha subcomplex-1), demonstrate significant downregulation in the presence of ADSCP2. Further analysis using the integrative genomics viewer indicates that the promoter regions of both COX6B1 and NDUFA1 exhibit a higher degree of closure in the ADSCP2 treatment group. Quantitative PCR analysis demonstrated that ADSCP2 treatment resulted in a reduction of COX6B1 and NDUFA1 mRNA expression levels. Furthermore, cellular ATP and lactic acid concentrations were diminished in the ADSCP2-treated group. Collectively, these findings suggest potential avenues for future research into the therapeutic application of the peptide ADSCP2 in the treatment of hypertrophic scars.

Herein, we describe three new species of the spider genus Naphrys Edwards, 2003 from Mexico: Naphrys echeri sp. nov., Naphrys tecoxquin sp. nov., and Naphrys tuuca sp. nov. An integrative taxonomic approach was applied, utilizing data from morphology, ultra-morphology, the mitochondrial gene COI, and distribution records. Four molecular methods for species delimitation were implemented under the corrected p-distance Neighbor-Joining (NJ) criteria: (1) Assemble Species by Automatic Partitioning (ASAP); (2) general mixed Yule coalescent (GMYC); (3) Bayesian Poisson tree process (bPTP); and (4) multi-rate Poisson tree process (mPTP). Both morphological and molecular data supported the delimitation and recognition of the three new species. The average interspecific genetic distance (p-distance) within the genus Naphrys is 14%, while the intraspecific genetic distances (p-distance) is <2% for most species. We demonstrate that the natural distribution of Naphrys is not restricted to the Nearctic region. Furthermore, the reported localities herein represent the first with precise locations in the country for Naphrys acerba. In addition, a taxonomic identification key is provided for the species in the genus.

Background: In the present scenario, competitive sports require athletes to achieve a phenomenal balance between cognitive abilities, motor skills, nutritional intake, and energy deficiencies. Such stability would enable the athletes to excel in their sporting field. Evidence shows that athletes develop specific cognitive abilities based on their sporting field. Nutrition is vital in creating an athlete's cognitive ability and physical needs required to participate in competitive sports. The reduction in the intake of nutrients required before, after and during sports participation could result in relative energy deficiency in sports (RED-S), affecting the parts of the body.

Methods: The rationale behind the survey is to understand the role of nutrition and energy deficiency on the athletes' cognitive abilities. The review's research areas were identified as athletes' cognition and nutrition in the context of RED-S. Search keywords were found based on the research area, such as "cognitive", "nutrition", and "energy deficiency/availability" in athletes. The search keywords were combined to form search queries (SQs). SQs were used to carry out the search on the Web of Science and Scopus databases.

Results: Sports play an important role in athletes' cognitive abilities, such as decision-making, attention, memory, etc. Nutritional intakes, such as caffeinated, carbohydrate, alkaline, and protein-based supplements and diets, also significantly affect athletes' cognitive and motor abilities. Low energy availability (LEA) causes cognitive and physical health problems in both female and male athletes.

Conclusion: The review identified that nutrition and LEA play crucial roles in athletes' cognitive performance. Deficits in nutritional intake and energy availability lead to RED-S. Hence, cognitive performance could be used as an early indication to identify the nutritional and energy deficits in advance, enabling athletes to combat RED-S.