The control effect of endophytic fungus Fusarium equiseti FUS-8 on cotton Verticillium wilt and its effects on soil microbial communities

IF 3.9 2区农林科学 Q1 AGRONOMY Plant and Soil Pub Date : 2024-12-17 DOI:10.1007/s11104-024-07141-4

Yun Zhang, Chao Xue, Xin Wang, Jianhua Zhang, Aiyu Wang, Yuanxue Yang, Xi Jia, Wenjuan Zhang, Ming Zhao

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Abstract

Aims

Cotton Verticillium wilt is a very serious soil-borne disease caused mainly by Verticillium dahliae, which severely affects the yield and quality of cotton. There has been an increasing amount of research on the biocontrol of plant disease; however, there is still a lack of effective biocontrol strains.

Methods

In this study, antagonistic endophytic fungi were screened to inhibit V. dahliae, and soil microbiome technologies were used to characterize the biocontrol mechanism.

Results

The strain FUS-8 was isolated from cotton stems; the strain significantly inhibited the growth of V. dahliae and was identified as Fusarium equiseti. FUS-8 had a small amount of cellulase activity, and its fermentation broth significantly inhibited the sporulation and colony formation of V. dahliae. After inoculation with FUS-8 in the greenhouse and field, the disease index significantly decreased. Preinoculation with FUS-8 inhibited the expression of pod. FUS-8 colonized the roots, hypocotyls, and stems of cotton. After preinoculation with FUS-8 in the greenhouse, the Shannon and Sobs indices of fungi and bacteria significantly decreased. The abundance of Bacillus genus in the treatment group was significantly greater than that in the control group 4 days after preinoculation with FUS-8 and 14 days after inoculation with V991. The LDA value of Bacillus in the treatment group was the highest at the genus level after inoculation with FUS-8 only; after reinoculation with V991, Pseudoxanthomonas had the highest LDA value in the treatment group.

Conclusion

FUS-8 altered soil microbial communities, enriched beneficial bacteria, and reduced the incidence of cotton Verticillium wilt.

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内生真菌马镰刀菌 FUS-8 对棉花轮纹枯萎病的防治效果及其对土壤微生物群落的影响

目的棉花轮纹病是一种非常严重的土传病害，主要由大丽轮纹霉菌（Verticillium dahliae）引起，严重影响棉花的产量和质量。本研究筛选了抑制大丽轮枝菌的拮抗内生真菌，并利用土壤微生物组技术研究了其生物防治机制。FUS-8 具有少量纤维素酶活性，其发酵液能显著抑制大丽花镰刀菌的孢子和菌落形成。在温室和田间接种 FUS-8 后，病害指数明显下降。FUS-8 的预接种抑制了荚果的表达。FUS-8 在棉花的根、下胚轴和茎上定殖。在温室中预先接种 FUS-8 后，真菌和细菌的 Shannon 和 Sobs 指数明显下降。在预接种 FUS-8 4 天后和接种 V991 14 天后，处理组的枯草芽孢杆菌属丰度明显高于对照组。结论 FUS-8 改变了土壤微生物群落，丰富了有益菌群，降低了棉花轮纹枯萎病的发病率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.