Richard Johannes Wierichs, Mowliharan Kuruparan, Abinaya Ruthiraswaran, Hendrik Meyer-Lueckel, Thiago Saads Carvalho, Samira Helena Niemeyer
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引用次数: 0
Abstract
Introduction: The aim of the study was to investigate the caries-preventive effect of fluoride-free toothpastes, containing either herbal agents or (nano-)hydroxyapatite.
Methods: Bovine dentin specimens each having a sound (ST) and a demineralized area (DT) were prepared and randomly allocated to eleven groups (n = 187). Treatments during pH cycling (28 days; 6 × 120 min demineralization/day) were brushing 2×/day with 0 ppm F- [NaF0], 500 ppm F- [NaF500], 1,100 ppm F- [NaF1100], grape seed extract [GSE], (nano-)hydroxyapatite, melaleuca oil [MO1, MO2, MO-CU], and propolis + myrrh [PM1, PM2] containing dentifrices. Dentifrice slurries were prepared with deionized water (1:3 w/w). Differences in integrated mineral loss (∆∆Z) and lesion depth (∆LD) were evaluated before and after pH cycling using transversal microradiography.
Results: The correlation between ΔΔZ/ΔLD and F- concentration (NaF0, NaF500, NaF1100) was strong for the DT (rΔΔZ, DT = 0.681; p < 0.001) and very strong for ST (rΔΔZ, ST = 0.861; p < 0.001), indicating a fluoride dose-response for both baseline substrate conditions. For ΔΔZDT and ΔLDDT, only NaF1100 and GSE revealed significant differences compared with NaF0 (p < 0.001; ANOVA). For ΔΔZST and ΔLDST, significant differences could be found for NaF1100 and NaF500 compared to all fluoride-free groups (p ≤ 0.002; ANOVA), without significant difference between fluoride-free groups (p = 1.000; ANOVA). For DT and ST, a hypermineralized surface layer and no surface loss could only be observed when fluoride was present.
Conclusion: A dose-response for fluoride concentrations was observed in this mild demineralization pH-cycling model. Fluoride-free dentifrices containing GSE or melaleuca oil showed certain preventive effect against further progression of root caries lesions. However, surface loss was observed for all fluoride-free dentifrices.
期刊介绍：
''Caries Research'' publishes epidemiological, clinical and laboratory studies in dental caries, erosion and related dental diseases. Some studies build on the considerable advances already made in caries prevention, e.g. through fluoride application. Some aim to improve understanding of the increasingly important problem of dental erosion and the associated tooth wear process. Others monitor the changing pattern of caries in different populations, explore improved methods of diagnosis or evaluate methods of prevention or treatment. The broad coverage of current research has given the journal an international reputation as an indispensable source for both basic scientists and clinicians engaged in understanding, investigating and preventing dental disease.
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