Structure and degradation dynamics of dietary pectin.

Abstract

Pectin, a complex dietary fiber, constitutes a key structural component of the cell walls of numerous edible plant products. It is resistant to digestion by human enzymes and undergoes depolymerization and saccharification in the gastrointestinal tract through the action of carbohydrate-active enzymes (CAZymes) produced by gut microbiota. This enzymatic breakdown generates intermediate structural fragments, which are subsequently converted into pectin oligosaccharides (POS) and monosaccharides. POS exhibit prebiotic properties and have demonstrated potential health benefits, including anti-carcinogenic effects, mucoadhesive capabilities, and the promotion of beneficial gut bacterial growth. However, the current understanding of the molecular structure of pectin and its degradation dynamics remains fragmented within the literature, impeding progress in dietary fiber intervention research and the development of personalized nutrition approaches. This review aims to provide a comprehensive overview of the structural features of pectin and the intricate breakdown mechanisms orchestrated by CAZymes. It underscores the complex architecture of pectin that influences its breakdown dynamics and specifies the enzymatic requirements for the cleavage of its diverse structural components. These insights complement our accompanying review on the structure-function relationships between pectin and the human gut microbiota, previously published in this journal.