Stability of Respiratory Syncytial Virus in Nasal Aspirate From Patients Infected With RSV

IF 4.3 4区医学 Q1 INFECTIOUS DISEASES Influenza and Other Respiratory Viruses Pub Date : 2024-12-16 DOI:10.1111/irv.70058

Atsuko Yamamoto, Yoko Hayasaki-Kajiwara, Takamichi Baba, Saori Okaga, Mayumi Kakui, Takao Shishido

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Abstract

Background

Evaluation of infectious virus titer is a challenge for respiratory syncytial virus (RSV) clinical trials because of the labile nature of RSV and rapid loss of infectivity without proper specimen handling. However, there has been no rigorous investigation into RSV stability in clinical specimens.

Methods

RSV stability was investigated by evaluating virus titers and defined as titer variation from baseline within three standard deviations of our titration assay. RSV stability in viral transport medium (VTM) at 4°C and the effect of freezing method on stability were evaluated using RSV-A2 stock. RSV stability in nasal aspirates collected in VTM at 4°C was estimated by regression analysis of virus titers measured at several time points. Stability of these specimens stored at −80°C for 10–15 months after freezing by the method, which maintained RSV-A2 stability, was also assessed.

Results

Three standard deviations were calculated from our titration assay as 0.97 log₁₀ 50% tissue culture infectious dose (TCID₅₀/mL), and RSV stability was defined as variation of virus titer from baseline within 1.0 log₁₀TCID₅₀/mL. RSV-A2 in VTM at 4°C was stable for at least 120 h. Freezing at −80°C negatively affected virus stability, whereas freezing in liquid nitrogen or a dry ice-ethanol bath did not. RSV in nasal aspirates was stable for 2 days at 4°C and for 10–15 months at −80°C after snap freezing.

Conclusions

RSV in nasal aspirates in VTM was estimated to be stable for 2 days at 4°C and for approximately 1 year at −80°C.

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RSV患者鼻吸物中呼吸道合胞病毒的稳定性。

背景：由于呼吸道合胞病毒（RSV）的不稳定性和不适当的标本处理，传染性病毒滴度的评估是临床试验的一个挑战。然而，尚未对临床标本中RSV的稳定性进行严格的调查。方法：通过评估病毒滴度来研究RSV的稳定性，并将其定义为滴度在我们的滴定法的三个标准偏差内与基线的滴度变化。采用RSV- a2原液，研究了RSV在4℃条件下在病毒转运介质（VTM）中的稳定性，以及冷冻方法对稳定性的影响。通过对多个时间点测量的病毒滴度进行回归分析，估计在4°C时采集的VTM鼻吸液中RSV的稳定性。在冷冻后-80°C保存10-15个月的样品的稳定性也被评估，以保持RSV-A2的稳定性。结果：我们的滴定试验计算出3个标准差为0.97 log10 50%组织培养感染剂量（TCID50/mL），定义RSV稳定性为病毒滴度在1.0 log10TCID50/mL范围内与基线的变化。RSV-A2在4°C的VTM中至少稳定120 h。在-80°C冷冻会对病毒的稳定性产生负面影响，而在液氮或干冰-乙醇浴中冷冻则不会。鼻吸液中的RSV在4°C条件下稳定2天，在-80°C条件下快速冷冻后稳定10-15个月。结论：据估计，VTM患者鼻吸液中的RSV在4°C下稳定2天，在-80°C下稳定约1年。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Influenza and Other Respiratory Viruses 医学-病毒学

CiteScore

7.20

自引率

4.50%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Influenza and Other Respiratory Viruses is the official journal of the International Society of Influenza and Other Respiratory Virus Diseases - an independent scientific professional society - dedicated to promoting the prevention, detection, treatment, and control of influenza and other respiratory virus diseases. Influenza and Other Respiratory Viruses is an Open Access journal. Copyright on any research article published by Influenza and Other Respiratory Viruses is retained by the author(s). Authors grant Wiley a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.