Genome-wide identification and expression analyses of FKBP and CYP gene family under salt and heat stress in Setaria italica L.

Abstract

As components of a family of proteins with peptidyl-prolyl isomerase activity family, FKBP (FK506-binding protein) and CYP (Cyclophilins) exert crucial roles in various physiological and biochemical processes such as cell signal transduction and stress resistance. The functions of the FKBP or CYP family have been extensively discussed in various organisms, while the comprehensive characterization of this family in Setaria italica remains unreported. In this study, a total of 22 SiFKBPs and 26 SiCYPs genes were identified in the genome of Setaria italica, with highly conserved functional domains observed within each member of these gene families. Phylogenetic analysis revealed that both FKBP and CYP proteins from Setaria italica and other plant species clustered into nine distinct groups. Furthermore, RT-qPCR results indicated that certain genes were induced specifically under salt stress while others were induced under heat stress, suggesting their involvement in stress response processes. The analysis of gene function revealed that SiFKBP16-3 exhibits some degree of functional conservation.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-024-01530-w.