In Vivo Contactless, Cellular-Resolution Imaging of the Healthy and Pathological Human Limbus With 250-kHz Point-Scanning SD-OCT.

IF 2.6 3区医学 Q2 OPHTHALMOLOGY Translational Vision Science & Technology Pub Date : 2024-12-02 DOI:10.1167/tvst.13.12.29

Kostadinka Bizheva, Zohreh Hosseinaee, Kirsten Carter, Denise Hileeto, Brian G Ballios, Luigina Sorbara, Hall F Chew

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Abstract

Purpose: To demonstrate that high-seed, ultra-high-resolution spectral-domain optical coherence tomography (SD-OCT) technology can image in vivo fine morphological features in the healthy and pathological human limbus.

Methods: A compact, fiberoptic SD-OCT system was developed for imaging the human limbus. It combines ∼1.5-µm isotropic spatial resolution in ocular tissue and an acquisition rate of 250,000 A-scans per second. The imaging probe was outfitted with two microscope objectives to provide flexibility in the choice of wide field of view and extended depth of focus versus high lateral resolution. The clinical potential of the system was evaluated by imaging subjects with limbal stem cell dysfunction (LSCD; n = 4) and healthy controls (n = 6).

Results: Limbus images acquired from the healthy controls showed normal cellular structure of the limbal crypts, palisades of Vogt (POVs), and vasculature of the underlying scleral tissue. Images acquired from the LSCD subjects showed distortions or absence of POVs, invasion of highly scattering conjunctival tissue over the limbal and peripheral corneal epithelium, scarring and thinning of the limbal epithelium, and neovascularization.

Conclusions: The combination of high OCT spatial resolution and rapid image acquisition rate allows for in vivo, contactless, volumetric visualization of fine morphological details that could be beneficial for the precise diagnosis and grading of LSCD, planning of treatment, and evaluation of the effectiveness of the treatment approaches.

Translational relevance: The OCT technology described here could improve the clinical diagnostics and grading of LSCD, preoperative planning, and postoperative evaluation of LSCD subjects, in addition to monitoring the effectiveness of various LSCD treatments.

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250 khz点扫描SD-OCT对健康和病理人体边缘的体内非接触、细胞分辨率成像

目的：证明高种子、超高分辨率光谱域光学相干断层扫描（SD-OCT）技术可以成像健康和病理人体边缘的体内精细形态学特征。方法：开发了一种紧凑的光纤SD-OCT系统，用于人体边缘成像。它结合了眼组织中约1.5µm的各向同性空间分辨率和每秒250,000次a扫描的采集速率。成像探针配备了两个显微镜物镜，以提供灵活的选择，宽视场和扩展聚焦深度与高横向分辨率。该系统的临床潜力是通过对患有角膜缘干细胞功能障碍(LSCD；n = 4)和健康对照（n = 6）。结果：健康对照获得的角膜边缘图像显示角膜边缘隐窝细胞结构、Vogt栅栏（pov）和底层巩膜组织血管结构正常。从LSCD受试者获得的图像显示pov扭曲或缺失，角膜缘和周围角膜上皮高度分散的结膜组织侵犯，角膜缘上皮瘢痕和变薄，以及新生血管。结论：高OCT空间分辨率和快速图像采集率的结合，可以实现活体、非接触、体积可视化的精细形态学细节，有助于LSCD的精确诊断和分级、治疗计划和治疗方法的有效性评估。翻译相关性：本文描述的OCT技术可以改善LSCD的临床诊断和分级，术前计划和术后评估LSCD受试者，以及监测各种LSCD治疗的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Vision Science & Technology Engineering-Biomedical Engineering

CiteScore

5.70

自引率

3.30%

发文量

346

审稿时长

25 weeks

期刊介绍： Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.