In-Situ Photoreaction Quantum Yield Measurements and Red-Light Actinometry Using Methylene Blue and Ascorbic Acid: Experiments for Analytical and Physical Chemistry

Abstract

In an effort to keep undergraduate chemical education at pace with contemporary interest in photochemical methodologies, we have identified a straightforward system for which steady-state photochemical kinetics may can be tracked via in situ UV–vis spectroscopy: an aqueous solution of methylene blue and ascorbic acid. Under red-light illumination, methylene blue forms a highly oxidizing excited state which is reduced by ascorbic acid, resulting in the colorless leucomethylene blue and the loss of methylene blue spectral features. Using a laser diode of steady output power, the quantum yield of the photoreaction was experimentally determined to be Φ_rxn = 0.106─indicating that about one in ten absorbed photons results in a productive reaction in this system. Φ_rxn is a critical metric in developing efficient photochemical reactions. The reaction was then used as a chemical actinometer to measure the power of an inexpensive red laser pointer and LED. These facile experiments can be employed in analytical and physical chemistry courses to expose students to important photochemical concepts through the familiar lens of UV–vis spectroscopy.