Determination of 31 Antimicrobials in Human Serum Using Ultra-High Performance Liquid Chromatography With Diode Array Detection for Application in Therapeutic Drug Monitoring.

IF 2.8 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY Therapeutic Drug Monitoring Pub Date : 2024-12-17 DOI:10.1097/FTD.0000000000001284

Ibrahim El-Haffaf, Mehdi El Hassani, Amélie Marsot

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Abstract

Background: A versatile ultra-high performance liquid chromatography method with diode array detection was developed to quantify a wide range of antibiotics in human serum. This method addresses the need for rapid and accurate determination of antibiotic levels to ensure effective patient treatment and support the fight against antibiotic resistance.

Methods: This method assesses 31 different compounds covering β-lactams, fluoroquinolones, antifungals, antituberculars, and more. Proteins were precipitated using methanol or acetonitrile, and drugs were extracted by liquid-liquid extraction with dichloromethane. Separation of the antimicrobials was achieved on a pentafluorophenyl column, using a mobile phase of phosphoric acid (0.01 mol/L) and acetonitrile in a gradient elution mode, with a flow rate of 500 μL/min.

Results: Almost all compounds were detected at 200 nm. The total analysis time for this method was kept under 18 minutes, including equilibration time.

Conclusions: This efficient method enables fast determination of numerous antimicrobial classes, providing clinicians with an essential tool for ensuring effective patient treatment and combating antimicrobial resistance.

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超高效液相色谱-二极管阵列检测在治疗药物监测中的应用

背景：建立了一种多功能的二极管阵列检测的超高效液相色谱法，用于定量测定人血清中的多种抗生素。该方法解决了快速准确测定抗生素水平的需求，以确保有效的患者治疗并支持对抗抗生素耐药性。方法：该方法评估了31种不同的化合物，包括β-内酰胺类、氟喹诺酮类、抗真菌药、抗结核药等。蛋白质用甲醇或乙腈沉淀，药物用二氯甲烷液液萃取。采用五氟苯基色谱柱，流动相为磷酸（0.01 mol/L）和乙腈，梯度洗脱，流速为500 μL/min。结果：几乎所有化合物均在200 nm处检出。该方法的总分析时间保持在18分钟以下，包括平衡时间。结论：这种有效的方法能够快速测定多种抗菌素类别，为临床医生提供了确保有效治疗患者和对抗抗菌素耐药性的重要工具。

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来源期刊

Therapeutic Drug Monitoring 医学-毒理学

CiteScore

5.00

自引率

8.00%

发文量

213

审稿时长

4-8 weeks

期刊介绍： Therapeutic Drug Monitoring is a peer-reviewed, multidisciplinary journal directed to an audience of pharmacologists, clinical chemists, laboratorians, pharmacists, drug researchers and toxicologists. It fosters the exchange of knowledge among the various disciplines–clinical pharmacology, pathology, toxicology, analytical chemistry–that share a common interest in Therapeutic Drug Monitoring. The journal presents studies detailing the various factors that affect the rate and extent drugs are absorbed, metabolized, and excreted. Regular features include review articles on specific classes of drugs, original articles, case reports, technical notes, and continuing education articles.