A non-invasive model for diagnosis of primary Sjogren’s disease based on salivary biomarkers, serum autoantibodies, and Schirmer’s test

IF 4.9 2区 医学 Q1 Medicine Arthritis Research & Therapy Pub Date : 2024-12-19 DOI:10.1186/s13075-024-03459-7
Xinwei Zhang, Zhangdi Liao, Yangchun Chen, Huiqin Lu, Aodi Wang, Yingying Shi, Qi Zhang, Ying Wang, Yan Li, Jingying Lan, Chubing Chen, Chaoqiong Deng, Wuwei Zhuang, Lingyu Liu, Hongyan Qian, Shiju Chen, Zhibin Li, Guixiu Shi, Yuan Liu
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Abstract

Minor salivary gland (MSG) biopsy is a critical but invasive method for the classification of primary Sjögren’s disease (pSjD). Here we aimed to identify salivary proteins as potential biomarkers and to establish a non-invasive prediction model for pSjD. Liquid chromatography-tandem mass spectrometry was conducted on whole saliva samples from patients with pSjD and non-Sjögren control subjects (non-pSjD). Proteins involved in immune processes were upregulated in the pSjD group, such as complement C3 (C3), complement factor B (CFB), clusterin (CLU), calreticulin (CALR), and neutrophil elastase (NE), which were further confirmed by ELISA. Multivariate logistic regression analyses were performed to identify markers that differentiated pSjD from non-pSjD; receiver operating characteristic (ROC) curves were constructed. A diagnostic model based on the combination of salivary biomarkers (CFB, CLU, and NE), serum autoantibodies (anti-SSA /Ro60 and anti-SSA/Ro52), and Schirmer’s test was evaluated in 186 patients (derivation cohort) with replication in 72 patients (validation cohort). In multivariate analyses, CFB, CLU, and NE were independent predictors of pSS. A model based on the combination of salivary biomarkers (CFB, CLU, and NE), serum autoantibodies (anti-SSA and anti-Ro52), and Schirmer’s test achieved significant discrimination of pSS. In the derivation cohort, the area under curve (AUC) of the ROC was 0.930 (95% CI 0.877–0.965, P < 0.001), with a sensitivity and specificity of 84.85% and 92.45%, respectively. Notably, similar results were obtained in a validation cohort. The 6-biomarker panel could provide a novel non-invasive tool for the classification of pSjD.
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基于唾液生物标志物、血清自身抗体和Schirmer试验的原发性干燥病诊断的无创模型
小唾液腺(MSG)活检是原发性Sjögren病(pSjD)分类的一种关键但有创的方法。本研究旨在鉴定唾液蛋白作为潜在的生物标志物,并建立pSjD的非侵入性预测模型。采用液相色谱-串联质谱法对pSjD患者和non-Sjögren对照组(非pSjD)的全唾液样本进行分析。pSjD组参与免疫过程的蛋白如补体C3 (C3)、补体因子B (CFB)、聚簇蛋白(CLU)、钙网蛋白(CALR)和中性粒细胞弹性酶(NE)上调,ELISA进一步证实了这一点。进行多变量logistic回归分析,以确定区分pSjD与非pSjD的标记;构建受试者工作特征(ROC)曲线。基于唾液生物标志物(CFB、CLU和NE)、血清自身抗体(抗ssa /Ro60和抗ssa /Ro52)和Schirmer试验的诊断模型在186例患者(衍生队列)中进行了评估,并在72例患者(验证队列)中进行了复制。在多变量分析中,CFB、CLU和NE是pSS的独立预测因子。基于唾液生物标志物(CFB、CLU和NE)、血清自身抗体(抗ssa和抗ro52)和Schirmer检验联合建立的模型对pSS有显著的鉴别效果。衍生队列的ROC曲线下面积(AUC)为0.930 (95% CI 0.877 ~ 0.965, P < 0.001),敏感性为84.85%,特异性为92.45%。值得注意的是,在验证队列中获得了类似的结果。6-生物标志物面板可为pSjD的分类提供一种新的无创工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.60
自引率
2.00%
发文量
261
审稿时长
14 weeks
期刊介绍: Established in 1999, Arthritis Research and Therapy is an international, open access, peer-reviewed journal, publishing original articles in the area of musculoskeletal research and therapy as well as, reviews, commentaries and reports. A major focus of the journal is on the immunologic processes leading to inflammation, damage and repair as they relate to autoimmune rheumatic and musculoskeletal conditions, and which inform the translation of this knowledge into advances in clinical care. Original basic, translational and clinical research is considered for publication along with results of early and late phase therapeutic trials, especially as they pertain to the underpinning science that informs clinical observations in interventional studies.
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