Molecular detection of Chlamydia psittaci in birds: a systematic review.

IF 2.5 2区 农林科学 Q1 VETERINARY SCIENCES Avian Pathology Pub Date : 2025-01-09 DOI:10.1080/03079457.2024.2443952
Xue Qi Soon, Kristene Gedye, Jackie Benschop, Brett Gartrell
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Abstract

Research highlights: Hierarchical molecular testing is recommended for the detection of avian C. psittaci.Key molecular tests for surveillance were conventional PCR and quantitative PCR.The most used genomic target to detect C. psittaci in birds was the ompA gene.

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鸟类鹦鹉热衣原体分子检测的系统综述。
分子方法是目前检测鸟类鹦鹉热衣原体最灵敏的方法。大多数实验室都开发了自己的分子分析方法或改编了已发表的方案,经常进行轻微修改以适应其特定的研究目的。分子检测的敏感性和特异性取决于靶基因、引物序列、分子检测类型、DNA提取方法和取样方法。我们回顾了2000年至2020年间发表的120篇论文,收集了鸟类鹦鹉热锥虫的分子检测信息。在目前可用于检测鸟类鹦鹉螺的10个基因组靶点中,ompA基因是应用最广泛的。在已发表的14种分子检测类型中,常规PCR和定量PCR应用最多。建议采用一种分层方法,包括对属和种特异性靶点进行分子测试,以检测除公认的鹦鹉螺外的其他鸟类衣原体物种。为了获得更好的准确性,样本应尽可能同时取自呼吸道和胃肠道。在最佳培养基和温度下保存样品,采用优化的DNA提取方案,可获得高质量的DNA。需要对分子衣原体检测进行标准化和验证,以提高检测鸟类鹦鹉热衣原体和其他衣原体的方法的可比性和可靠性。禽鹦鹉热的分级分子检测是一种有效的检测方法。监测的主要分子检测方法为常规PCR和定量PCR。在鸟类中检测鹦鹉螺杆菌最常用的基因组靶点是ompA基因。
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来源期刊
Avian Pathology
Avian Pathology 农林科学-兽医学
CiteScore
4.50
自引率
10.70%
发文量
68
审稿时长
1 months
期刊介绍: Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.
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