Transcriptomic analysis reveals three important carbohydrate-active enzymes contributing to starch degradation of oleaginous yeast Lipomyces starkeyi.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-18 DOI:10.1093/bbb/zbae199

Kentaro Mine, Hiroya Taki, Juyoung Kim, Jiro Seto, Shinji Matsuo, Rikako Sato, Hiroaki Takaku

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Abstract

The oleaginous yeast Lipomyces starkeyi has a high capacity for starch assimilation, but the genes involved and specific mechanisms in starch degradation remain unclear. This study aimed to identify the critical carbohydrate-active enzyme (CAZyme) genes contributing to starch degradation in L. starkeyi. Comparative transcriptome analysis of cells cultured in glucose and soluble starch medium revealed that 55 CAZymes (including transcript IDs 3772, 1803, and 7314) were highly expressed in soluble starch medium. Protein domain structure and disruption mutant analyses revealed that 3772 encodes the sole secreted α-amylase (LsAmy1p), whereas 1803 and 7314 encode secreted α-glucosidase (LsAgd1p and LsAgd2p, respectively). Triple-gene disruption exhibited severely impaired growth in soluble starch, dextrin, and raw starch media, highlighting their critical role in degrading polysaccharides composed of glucose linked by α-1,4-glucosidic bonds. This study provided insights into the complex starch degradation mechanism in L. starkeyi.

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转录组学分析揭示了三种重要的碳水化合物活性酶对产油酵母淀粉降解的贡献。

产油酵母starkeyi脂酵母具有较高的淀粉同化能力，但淀粉降解的相关基因和具体机制尚不清楚。本研究旨在鉴定淀粉降解的关键碳水化合物活性酶（CAZyme）基因。在葡萄糖和可溶性淀粉培养基中培养的细胞转录组对比分析显示，55个CAZymes（包括转录物id 3772、1803和7314）在可溶性淀粉培养基中高表达。蛋白结构域结构和断裂突变分析表明，3772编码唯一分泌α-淀粉酶（LsAmy1p），而1803和7314编码α-葡萄糖苷酶（LsAgd1p和LsAgd2p）。在可溶性淀粉、糊精和生淀粉培养基中，三基因断裂表现出严重的生长受损，突出了它们在降解由α-1,4-糖苷键连接的葡萄糖组成的多糖中的关键作用。本研究为揭示星花草复杂淀粉降解机制提供了新的思路。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).