N6-methyladenosine on the natural antisense transcript of NIA1 stabilizes its mRNA to boost NO biosynthesis and modulate stomatal movement.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Plant Pub Date : 2024-12-17 DOI:10.1016/j.molp.2024.12.011
Jie Li, Wen Tian, Ting Chen, Qing-Yan Liu, Hua-Wei Wu, Chuan-Hui Liu, Yuan-Yuan Fang, Hui-Shan Guo, Jian-Hua Zhao
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引用次数: 0

Abstract

Nitric oxide (NO) is a crucial signaling molecule that regulates a wide range of metabolic pathways in different strata of organisms. In plants, nitrate reductase (NR) is a key enzyme for NO biosynthesis. There are two NR-encoding genes in Arabidopsis, NIA1 and NIA2, which are precisely regulated and expressed in a tissue-specific manner. Here, we found that the natural antisense transcript as-NIA1, transcribed from the 3' UTR of NIA1, stabilizes NIA1 mRNA to maintain its circadian oscillation in plants grown under the light/dark cycle. Importantly, as-NIA1-dependent NIA1 mRNA stability is indispensable for NIA1-mediated NO biosynthesis in guard cells and natural stomatal closure. Moreover, we revealed that PTB3 regulates the stabilization of NIA1 mRNA by directly binding to UC-rich elements of as-NIA1. We further found that MTA deposits N6-methyladenosine (m6A) on as-NIA1, facilitating the as-NIA1-PTB3 interaction in vivo, in agreement with RNA structure prediction in that m6A-mediated structural alterations expose the UC-rich elements whereby enhancing the accessibility of PTB3. Our findings reveal a mechanism by which plants precisely manipulate NO biosynthesis to modulate light/dark-regulated stomatal movement, expanding the understanding of the coupling of RNA epigenetic modifications and structures shaping RNA‒protein interactions.

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Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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