Prognostic analysis of SYTL4 in acute myeloid leukemia.

IF 1.5 4区医学 Q4 ONCOLOGY Translational cancer research Pub Date : 2024-11-30 Epub Date: 2024-11-12 DOI:10.21037/tcr-24-758

Kun-Ying Xie, Jin Wei

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Abstract

Background: Acute myeloid leukemia (AML) is a highly heterogeneous hematological malignancy. The key problem lies in the complexity of the genome, so that drug resistance and relapse have become the main problems. Recent studies have found an association between synaptotagmin-like 4 (SYTL4) and drug resistance in triple-negative breast cancer and its high expression is correlated with poor prognosis; however, it is unclear whether this gene is associated with the prognosis of AML. This study aimed to investigate the role and action mechanism of SYTL4 in AML.

Methods: We downloaded gene expression profiles and corresponding clinical data from The Cancer Genome Atlas (TCGA) public database and conducted differential and survival analyses using the Limma and survival packages in R. The receiver operating characteristic (ROC) curve, univariate COX, and multivariate COX were used for gene prediction analysis. Co-expression analysis of SYTL4 was performed using Limma, and enrichment analysis of differentially expressed genes in the SYTL4 high- and low-expression groups was conducted. We performed immune cell infiltration using the CIBERSORTx algorithm.

Results: The expression level of SYTL4 was highest in the poor prognosis group, and lowest in the good prognosis group. Survival was better in the SYTL4 low expression group than that in the high expression group. The areas under the ROC curve for TCGA-Acute Myeloid Leukemia (TCGA-LAML) at 1, 3, and 5 years were 0.725, 0.683, and 0.787, respectively. Sushi repeat protein X-linked 2 (SRPX2), caveolae associated protein 2 (CAVIN2), and other genes were identified as positive regulators of SYTL4 expression, whereas lactoperoxidase (LPO), diacylglycerol lipase beta (DAGLB), and other genes were identified as negative regulators. Differentially expressed genes in the SYTL4 high- and low-expression groups were enriched in pathways such as the embryonic skeletal system and platelet alpha granules. Differences were observed in follicular helper T cells, Tregs, monocytes, and M2 macrophages between SYTL4 high- and low-expression groups.

Conclusions: SYTL4 expression negatively correlates with AML prognosis and may be associated with exosome secretion in AML.

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SYTL4在急性髓系白血病中的预后分析。

背景：急性髓性白血病（AML）是一种高度异质性的血液系统恶性肿瘤。关键问题在于基因组的复杂性，使耐药和复发成为主要问题。近期研究发现SYTL4与三阴性乳腺癌耐药相关，且其高表达与预后不良相关；然而，目前尚不清楚该基因是否与AML的预后相关。本研究旨在探讨SYTL4在AML中的作用及作用机制。方法：从美国癌症基因组图谱（The Cancer Genome Atlas， TCGA）公共数据库下载基因表达谱及相应的临床数据，使用r中的Limma和survival软件包进行差异和生存分析。采用受试者工作特征（ROC）曲线、单因素COX和多因素COX进行基因预测分析。采用Limma软件进行SYTL4共表达分析，并对SYTL4高、低表达组差异表达基因进行富集分析。我们使用CIBERSORTx算法进行免疫细胞浸润。结果：SYTL4在预后不良组中表达量最高，在预后良好组中表达量最低。SYTL4低表达组生存率高于高表达组。tcga -急性髓系白血病（TCGA-LAML） 1、3、5年时的ROC曲线下面积分别为0.725、0.683、0.787。Sushi repeat protein X-linked 2 （SRPX2）、CAVIN2 （CAVIN2）等基因为SYTL4表达的正调控基因，而乳酸过氧化物酶（LPO）、二酰基甘油脂肪酶（DAGLB）等基因为SYTL4表达的负调控基因。SYTL4高表达组和低表达组的差异表达基因在胚胎骨骼系统和血小板α颗粒等途径中富集。在SYTL4高表达组和低表达组中，滤泡辅助性T细胞、treg细胞、单核细胞和M2巨噬细胞均存在差异。结论：SYTL4表达与AML预后呈负相关，并可能与AML外泌体分泌有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational cancer research ONCOLOGY-

CiteScore

2.10

自引率

0.00%

发文量

252

期刊介绍： Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.