Effect of Alpinia officinarum Rhizome extract on experimentally induced lung fibrosis: The pertinent role of Sirt1 and Nrf2 antioxidant pathways.

Q3 Medicine Morphologie Pub Date : 2024-12-17 DOI:10.1016/j.morpho.2024.100940

Marwa A S Al-Gholam, Huda I Abd-Elhafiz, Sara G Tayel

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引用次数: 0

Abstract

Background: Pulmonary fibrosis (PF) is a frequently reported COVID-19 sequela. It is a progressive disorder characterized by respiratory failure and death. The properties of Alpinia officinarum Rhizomes (AO) make it a highly potent antioxidant, anti-inflammatory, and antifibrotic agent. This study has evaluated AO's protective effects on bleomycin-induced PF in rats and investigated the underlying mechanisms.

Material and methods: Bleomycin (5mg/kg, intratracheally) was used to induce PF in albino rats, and then, AO extract (200mg/kg/daily, orally) was administrated for 28days post-bleomycin-instillation. After euthanizing the rats, the biochemical, quantitative real-time polymerase chain reaction (qPCR) and histopathological examination of lung tissue were determined.

Results: Findings have revealed that bleomycin significantly increased the tissue level of malondialdehyde, tumor necrosis factor-alpha, and interleukin-6, Silent information regulator 1 (Sirt1), and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA levels. Furthermore, the total antioxidant capacity level decreased in the lungs of bleomycin-instilled rats. However, AO extract significantly decreased histopathological injuries in hematoxylin & eosin, Masson's trichrome-stained sections, inducible nitric oxide synthase and α-smooth muscle actin, transforming growth factor beta 1 immunoexpression.

Conclusion: Alpinia officinarum Rhizomes extract appears to protect against bleomycin-induced PF, possibly due to its antioxidant, anti-inflammatory, and antifibrotic properties.

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来源期刊

Morphologie Medicine-Anatomy

CiteScore

2.30

自引率

0.00%

发文量

150

审稿时长

25 days

期刊介绍： Morphologie est une revue universitaire avec une ouverture médicale qui sa adresse aux enseignants, aux étudiants, aux chercheurs et aux cliniciens en anatomie et en morphologie. Vous y trouverez les développements les plus actuels de votre spécialité, en France comme a international. Le objectif de Morphologie est d?offrir des lectures privilégiées sous forme de revues générales, d?articles originaux, de mises au point didactiques et de revues de la littérature, qui permettront notamment aux enseignants de optimiser leurs cours et aux spécialistes d?enrichir leurs connaissances.