Adipose tissue protects against skin photodamage through CD151- and AdipoQ- EVs.

IF 8.2 2区生物学 Q1 CELL BIOLOGY Cell Communication and Signaling Pub Date : 2024-12-18 DOI:10.1186/s12964-024-01978-z

Yan-Wen Wang, Poh-Ching Tan, Qing-Feng Li, Xue-Wen Xu, Shuang-Bai Zhou

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Abstract

To clarify the protective effects of subcutaneous adipose tissue (SAT) against photodamage, we utilized nude mouse skin with or without SAT. Skin and fibroblasts were treated with adipose tissue-derived extracellular vesicles (AT-EVs) or extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) to demonstrate that SAT protects the overlying skin from photodamage primarily through AT-EVs. Surprisingly, AT-EVs stimulated fibroblast proliferation more rapidly than ADSC-EVs did. The yield of AT-EVs from the same volume of AT was 200 times greater than that of ADSC-EVs. To compare the differences between AT-EVs and ADSC-EVs, we used a proximity barcoding assay (PBA) to analyze the surface proteins on individual particles of these two types of EVs. PBA analysis revealed that AT-EVs contain diverse subpopulations, with 83.42% expressing CD151, compared to only 1.98% of ADSC-EVs. Furthermore, AT-EVs are internalized more rapidly by cells than ADSC-EVs, as our study demonstrated that CD151-positive AT-EVs were endocytosed more quickly than their CD151-negative counterparts. Additionally, adiponectin in AT-EVs activated the AMPK pathway and inhibited the NF-κB pathway, enhancing fibroblast protection against photodamage. The significantly higher yield and faster acquisition of AT-EVs compared to ADSC-EVs underscore their potential for broader applications.

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脂肪组织通过CD151-和AdipoQ- ev保护皮肤免受光损伤。

为了阐明皮下脂肪组织（SAT）对光损伤的保护作用，我们使用了含有或不含有SAT的裸鼠皮肤。用脂肪组织来源的细胞外囊泡（at - ev）或脂肪来源的干细胞（adsc - ev）处理皮肤和成纤维细胞，以证明SAT主要通过at - ev保护覆盖的皮肤免受光损伤。令人惊讶的是，at - ev比adsc - ev更快地刺激成纤维细胞增殖。相同体积AT的AT- ev产量是adsc - ev的200倍。为了比较at - ev和adsc - ev的差异，我们使用了接近条形码分析（PBA）来分析这两种类型ev的单个颗粒表面蛋白。PBA分析显示at - ev含有不同的亚群，83.42%的at - ev表达CD151，而adsc - ev只有1.98%表达CD151。此外，at - ev比adsc - ev被细胞更快地内化，因为我们的研究表明，cd151阳性的at - ev比cd151阴性的at - ev被更快地内吞。此外，at - ev中的脂联素激活AMPK通路，抑制NF-κB通路，增强成纤维细胞对光损伤的保护作用。与adsc - ev相比，at - ev的产量明显更高，获取速度更快，这突显了它们具有更广泛应用的潜力。

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来源期刊

Cell Communication and Signaling CELL BIOLOGY-

CiteScore

11.00

自引率

0.00%

发文量

180

期刊介绍： Cell Communication and Signaling (CCS) is a peer-reviewed, open-access scientific journal that focuses on cellular signaling pathways in both normal and pathological conditions. It publishes original research, reviews, and commentaries, welcoming studies that utilize molecular, morphological, biochemical, structural, and cell biology approaches. CCS also encourages interdisciplinary work and innovative models, including in silico, in vitro, and in vivo approaches, to facilitate investigations of cell signaling pathways, networks, and behavior. Starting from January 2019, CCS is proud to announce its affiliation with the International Cell Death Society. The journal now encourages submissions covering all aspects of cell death, including apoptotic and non-apoptotic mechanisms, cell death in model systems, autophagy, clearance of dying cells, and the immunological and pathological consequences of dying cells in the tissue microenvironment.