Pingrui Xu, Yongshuang Xiao, Junde Dong, Zhizhong Xiao, Jun Li, Yanfeng Wang
{"title":"Rapid Sex Identification in Spotted Knifejaw (Oplegnathus punctatus) Using tmem88 Gene Structural Variation Markers","authors":"Pingrui Xu, Yongshuang Xiao, Junde Dong, Zhizhong Xiao, Jun Li, Yanfeng Wang","doi":"10.1007/s10126-024-10403-y","DOIUrl":null,"url":null,"abstract":"<div><p>Spotted knifejaw (<i>Oplegnathus punctatus</i>) is an economically important marine cultured species exhibiting a unique complex sex chromosome system (X<sub>1</sub>X<sub>1</sub>X<sub>2</sub>X<sub>2</sub> in females and X<sub>1</sub>X<sub>2</sub>Y in males), with males possessing one fewer chromosome (2n = 47) than females (2n = 48) and an abnormally large Y chromosome. Additionally, males demonstrate significant growth advantages over females. Rapid and accurate sex identification is essential for effective culture management, selective breeding, and population control. In this study, we identified a homologous region of the <i>tmem88</i> gene containing large DNA insertion markers on the X and Y chromosomes through whole-genome sequencing of <i>O. punctatus</i>. The X<sub>1</sub> chromosome harbors a 278 bp DNA fragment, whereas the Y chromosome contains a 1472 bp fragment, resulting in a 1194 bp size difference indicative of structural variation in the non-coding region of the <i>tmem88</i> gene. We developed a rapid detection method based on this variation, utilizing a pair of primers that amplify two distinct bands (278 bp and 1472 bp) in male (X<sub>1</sub>X<sub>2</sub>Y) individuals and a single 278 bp band in female (X<sub>1</sub>X<sub>1</sub>X<sub>2</sub>X<sub>2</sub>) individuals when analyzed by agarose gel electrophoresis. This method enables efficient and accurate sex differentiation in <i>O. punctatus</i>, significantly reducing the time required for identification and enhancing detection efficiency. This study provides a valuable tool for the rapid identification of sex in <i>O. punctatus</i>, facilitating improved breeding strategies and supporting the large-scale production of high-quality fry.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10403-y.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10126-024-10403-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Spotted knifejaw (Oplegnathus punctatus) is an economically important marine cultured species exhibiting a unique complex sex chromosome system (X1X1X2X2 in females and X1X2Y in males), with males possessing one fewer chromosome (2n = 47) than females (2n = 48) and an abnormally large Y chromosome. Additionally, males demonstrate significant growth advantages over females. Rapid and accurate sex identification is essential for effective culture management, selective breeding, and population control. In this study, we identified a homologous region of the tmem88 gene containing large DNA insertion markers on the X and Y chromosomes through whole-genome sequencing of O. punctatus. The X1 chromosome harbors a 278 bp DNA fragment, whereas the Y chromosome contains a 1472 bp fragment, resulting in a 1194 bp size difference indicative of structural variation in the non-coding region of the tmem88 gene. We developed a rapid detection method based on this variation, utilizing a pair of primers that amplify two distinct bands (278 bp and 1472 bp) in male (X1X2Y) individuals and a single 278 bp band in female (X1X1X2X2) individuals when analyzed by agarose gel electrophoresis. This method enables efficient and accurate sex differentiation in O. punctatus, significantly reducing the time required for identification and enhancing detection efficiency. This study provides a valuable tool for the rapid identification of sex in O. punctatus, facilitating improved breeding strategies and supporting the large-scale production of high-quality fry.
期刊介绍:
Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.