Identification of components that increase NAD+ levels in oxygen-glucose deprived HUVEC s from Schisandra chinensis (Turcz.) Baill. Based on spectrum-effect correlation analysis and target cell extraction

IF 2.5 3区 医学 Q3 CHEMISTRY, MEDICINAL Fitoterapia Pub Date : 2024-12-17 DOI:10.1016/j.fitote.2024.106347
Liwenyu Chen , Yi Yu , Min Lin , Ming Liang , Chen Yang , Xutian Kan , Xiaoliang Lin , Jin Qi
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Abstract

Schisandra chinensis (Turcz.) Baill, a traditional Chinese medicine with significant nourishing functions, has a regulatory effect on the cardiovascular system, digestive system, central nervous system, and endocrine and immune systems. It can protect the cardiovascular system, improve immunity, and has anti-oxidant and anti-aging properties. This study aimed to identify the chisandra chinensis components that increased NAD+ levels, by using spectrum-effect analysis and experimental validations. First, the quality of the S. chinensis extract was analyzed by HPLC-MS. The extract of S. chinensis increased the NAD+ levels of HUVECs during oxygen and glucose deprivation injury, and protected HUVEC s from injury from superoxide dismutase (SOD) and aging. The relationships of spectral effects were studied by partial least square regression. Coupled with target cell extraction, the material basis for increasing NAD+ levels in S. chinensis was obtained. The pharmacological activity of S. chinensis was verified at the cellular level. The related enzymes of the NAD+ synthesis and decomposition pathways were identified, showing that S. chinensis increased the level of NAD+ by increasing the activity of related enzymes in the synthesis pathways of NMNAT1, NMNAT2, NMNAT3, and NAMPT, but had no effect on the decomposition pathway. Finally, four constituents were confirmed, in vitro, to be the basis of S. chinensis-induced increases in the levels of NAD+. The EC50 values of the four constituents were also determined.

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五味子缺氧葡萄糖HUVEC中增加NAD+水平的成分鉴定(图)Baill。基于光谱效应相关分析和目标细胞提取。
五味子(土耳其)牛耳是一种具有显著滋补功能的中药,对心血管系统、消化系统、中枢神经系统、内分泌和免疫系统都有调节作用。它可以保护心血管系统,提高免疫力,并具有抗氧化和抗衰老的特性。本研究旨在通过光谱效应分析和实验验证,鉴定五味子中提高NAD+水平的成分。首先,采用高效液相色谱-质谱法对五味子提取物进行质量分析。五味子提取物可提高缺氧和葡萄糖剥夺损伤时HUVEC细胞NAD+水平,保护HUVEC细胞免受超氧化物歧化酶(SOD)损伤和衰老。利用偏最小二乘回归研究了光谱效应之间的关系。结合靶细胞的提取,获得了提高五味子NAD+水平的物质基础。在细胞水平上验证了五味子的药理活性。对NAD+合成和分解途径的相关酶进行了鉴定,结果表明,羊草通过提高NMNAT1、NMNAT2、NMNAT3和NAMPT合成途径中相关酶的活性来提高NAD+水平,但对分解途径没有影响。最后,在体外实验中,四种成分被证实是中国葡萄球菌诱导NAD+水平升高的基础。测定了四种成分的EC50值。
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Formic acid
来源期刊
Fitoterapia
Fitoterapia 医学-药学
CiteScore
5.80
自引率
2.90%
发文量
198
审稿时长
1.5 months
期刊介绍: Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.
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