Cell death by apoptosis induced by Ocotea indecora (Schott) Mez essential oil in different cancer cell lines.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2024-12-20 DOI:10.1080/14786419.2024.2443492

Francisco Paiva Machado, Ana Caroline Diniz-Dos-Santos, Lucas Nicolau de Queiroz, Caio Pinho Fernandes, Leandro Rocha, Bruno Kaufmann Robbs

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Abstract

Cancer is the second leading cause of death in the world. Plants from Ocotea genus have been studied for their biological activities. Here we evaluated the antitumor activity of Ocotea indecora essential oil in several cancer cell lines and its phytochemical composition. We performed cell viability using MTT (1000-31.25 μg/mL) using DMSO (negative) and carboplatin (positive) controls. Cytometric and morphological assays analysed cell death pathway. In vivo acute toxicity was assessed in mice (150, 300, and 900 mg/kg) and GCMC for phytochemical analysis. The oil showed sesquirosefuran as the main metabolite and was cytotoxic against all cell lines tested, mainly HEPG2 (human hepatoblastoma cell) (IC₅₀=44.9 µg/mL), was selective (SI = 1.69) and tolerated in mice. Apoptosis is the main cell death pathway as indicated by the presence of active caspase 3/7 and morphological characteristics. In conclusion, the oil was effective against HEPG2 cells and could be considered for future cancer studies.

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Ocotea indecora (Schott) Mez 精油在不同癌细胞系中诱导细胞凋亡。

癌症是世界上第二大死因。人们对茶属植物的生物活性进行了研究。本文研究了乌桕精油对几种肿瘤细胞系的抗肿瘤活性及其植物化学成分。以DMSO（阴性）和卡铂（阳性）为对照，采用MTT （1000-31.25 μg/mL）检测细胞活力。细胞术和形态学分析细胞死亡途径。小鼠体内急性毒性（150、300和900 mg/kg）和GCMC进行植物化学分析。该精油主要代谢产物为倍半玫瑰呋兰，对所有细胞系均有细胞毒性，主要是HEPG2（人肝母细胞瘤细胞）（IC50=44.9µg/mL），具有选择性（SI = 1.69），小鼠耐受。从活性caspase 3/7的存在和形态学特征可以看出，凋亡是细胞死亡的主要途径。综上所述，该油对HEPG2细胞有效，可用于未来的癌症研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.