[MMLV reverse transcriptase, a relevant tool for molecular biology].

IF 0.7 4区 医学 Q4 VIROLOGY Virologie Pub Date : 2024-12-20 DOI:10.1684/vir.2024.1070
Eva Lopez, Coralie Valle, Bruno Coutard
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引用次数: 0

Abstract

The reverse transcriptase of Moloney Murine Leukemia Virus (MMLV) is an enzyme that synthesizes DNA from an RNA template. Among reverse transcriptases, this enzyme is currently the most commonly used in molecular biology and diagnostics. Since its discovery, this viral protein has been extensively studied, shedding light on its structural and functional characteristics, and offering opportunities to optimize the catalytic performances for biotechnological applications. The review describes the structural motifs of the reverse transcriptase of MMLV, the key amino acids in enzymatic catalysis and the enzyme's properties such as processivity, fidelity and thermal stability. This review reports the optimizations made to improve these parameters, which enabled the modified enzymes to be integrated in the molecular biology tests used in the laboratory on a daily basis.

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[MMLV逆转录酶,分子生物学的相关工具]。
Moloney小鼠白血病病毒(MMLV)的逆转录酶是一种从RNA模板合成DNA的酶。在逆转录酶中,该酶是目前在分子生物学和诊断学中最常用的酶。自发现以来,人们对该病毒蛋白进行了广泛的研究,揭示了其结构和功能特征,并为优化生物技术应用的催化性能提供了机会。本文综述了MMLV逆转录酶的结构基序、酶催化的关键氨基酸以及该酶的活性、保真度和热稳定性等特性。本文综述了为改善这些参数而进行的优化,使改性酶能够集成到实验室日常使用的分子生物学测试中。
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来源期刊
Virologie
Virologie VIROLOGY-
CiteScore
1.20
自引率
22.20%
发文量
31
审稿时长
>12 weeks
期刊介绍: Virologie offers top-level information for all those for whom virology is already the main or an important part of their activity: biologists, clinicians, pharmacists, veterinarians, agronomists, etc.
期刊最新文献
[37th International Conference on Antiviral Research (ICAR)]. [Joint Thesis Award 2023 of the Société française de virologie and ANRS | Maladies infectieuses émergentes]. [XIXe atelier international sur les parvovirus (Leuven, Belgique), 03-06 septembre 2024]. [From viral disease gender to viral species subclassification: how to find our way around?] [Human papillomaviruses : origin, oncogenic factors and markers for cervical cancer screening].
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