{"title":"Investigation of Potential Food Intake Biomarkers by LC‐MS/MS: Suitability Under Conditions Close to Everyday Live","authors":"Amelie Frank, Florian Hübner, Hans‐Ulrich Humpf","doi":"10.1002/mnfr.202400575","DOIUrl":null,"url":null,"abstract":"ScopeFood intake biomarkers are proposed to be a more reliable tool for investigating the nutritional status compared to food diaries or food frequency questionnaires. Nevertheless, biomarkers have some disadvantages such as the influence of the individual lifestyle, different metabolism, nutrient–nutrient interaction or the specificity of the used biomarkers. To evaluate the suitability of several biomarkers, a study under conditions close to everyday life was conducted.Methods and resultsTen healthy volunteers were divided into three groups who followed different meal plans for 24 h and collected their entire urine. Afterward, several potential biomarkers were quantified using an LC‐MS/MS approach. To further investigate the suitability of those biomarkers, the results were investigated with the main goal of grouping the participants according to their provided diet, while the first author did not have any knowledge of the meal plans or other specific knowledge about the study design.ConclusionThe use of different biomarkers enables the evaluation of different eating habits without any prior wash out period or bigger than usual portion sizes. Especially long‐term biomarkers seem to have a good suitability. Very high intakes and very low intakes are easier to distinguish than a moderate intake. Food intake biomarkers are proposed as a more reliable tool than food diaries or food frequency questionnaires. Nevertheless, data under realistic conditions are rarely available. In this study, ten volunteers were divided into three groups with different meal plans. An LC‐MS/MS approach was performed and the data were further investigated with the main goal of grouping the participants according their provided diet, while the first author did not have any knowledge about the meal plans.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"31 1","pages":""},"PeriodicalIF":4.5000,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Nutrition & Food Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/mnfr.202400575","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
ScopeFood intake biomarkers are proposed to be a more reliable tool for investigating the nutritional status compared to food diaries or food frequency questionnaires. Nevertheless, biomarkers have some disadvantages such as the influence of the individual lifestyle, different metabolism, nutrient–nutrient interaction or the specificity of the used biomarkers. To evaluate the suitability of several biomarkers, a study under conditions close to everyday life was conducted.Methods and resultsTen healthy volunteers were divided into three groups who followed different meal plans for 24 h and collected their entire urine. Afterward, several potential biomarkers were quantified using an LC‐MS/MS approach. To further investigate the suitability of those biomarkers, the results were investigated with the main goal of grouping the participants according to their provided diet, while the first author did not have any knowledge of the meal plans or other specific knowledge about the study design.ConclusionThe use of different biomarkers enables the evaluation of different eating habits without any prior wash out period or bigger than usual portion sizes. Especially long‐term biomarkers seem to have a good suitability. Very high intakes and very low intakes are easier to distinguish than a moderate intake. Food intake biomarkers are proposed as a more reliable tool than food diaries or food frequency questionnaires. Nevertheless, data under realistic conditions are rarely available. In this study, ten volunteers were divided into three groups with different meal plans. An LC‐MS/MS approach was performed and the data were further investigated with the main goal of grouping the participants according their provided diet, while the first author did not have any knowledge about the meal plans.
期刊介绍:
Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines:
Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics.
Immunology: Understanding the interactions of food and the immune system.
Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes.
Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.