Biochemical and molecular mechanisms of Rhodococcus rhodochrous IITR131 for polyethylene terephthalate degradation.

Abstract

Aims: To isolate polyethylene terephthalate (PET)-degrading bacteria and elucidate the underlying mechanisms of PET biodegradation through biochemical and genome analysis.

Methods and results: Rhodococcus rhodochrous IITR131 was found to degrade PET. Strain IITR131 genome revealed metabolic versatility of the bacterium and had the ability to form biofilm on PET sheet, resulting in the cracks, abrasions, and degradation. IITR131 showed a reduction of 19.7%, exhibiting a half-life of 189.9 d of 0.1 mm PET film in 60 d and formed metabolites bis(2-hydroxyethyl) terephthalate (BHET), terephthalic acid (TPA), and benzoic acid (BA). The draft genome of 5.9 Mb of IITR131 revealed that this bacterium has plethora of genes such as terephthalate 1, 2 dioxygenase, carboxylesterase that together constituted a complete pathway for PET degradation. Moreover, strain IITR131 was found to have a variety of genes encoding for enzymes for the metabolism of several plastic polymers, xenobiotics including chloroalkanes, and polycyclic aromatic hydrocarbons.

Conclusions: Rhodococcus rhodochrous IITR131 demonstrated a significant potential in the biodegradation of PET. The comprehensive genomic and metabolic analyses further elucidated the molecular pathway involved in PET degradation, enhancing our understanding of the mechanisms underlying microbial PET biodegradation. These findings underscore the applicability of R. rhodochrous IITR131 in biotechnological approaches for mitigating plastic pollution.