Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension‐Culture Cells of Cyclocarya paliurus

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioengineering Pub Date : 2024-12-23 DOI:10.1002/bit.28913

Chuanqing Pan, Yuan Liu, Daobang Tang, Jiguang Chen, Zhongping Yin

{"title":"Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension‐Culture Cells of Cyclocarya paliurus","authors":"Chuanqing Pan, Yuan Liu, Daobang Tang, Jiguang Chen, Zhongping Yin","doi":"10.1002/bit.28913","DOIUrl":null,"url":null,"abstract":"Hormones are effective in regulating plant metabolism and, therefore, are often used in plant cell culture to increase the yield of target products. This study investigated the effects of hormones on the growth and anthocyanin biosynthesis of suspension‐cultured red Cyclocarya paliurus cells. Additionally, the mechanism by which gibberellin induces anthocyanin biosynthesis was explored through multi‐omics integrated analysis and the assay of the dynamic changes in signaling molecule concentration. The results showed that the total anthocyanin content and yield of suspension‐cultured cells, when induced by 1.0 mg L⁻¹ Gibberellin A3 (GA3), experienced increases of 1.92‐ and 1.83‐fold, respectively. The application of exogenous GA3 activated the synthesis and transduction of four signaling molecules, that is, nitric oxide (NO), hydrogen peroxide (H2O2), salicylic acid (SA), and jasmonic acid (JA), in the cells and altered the expression patterns of transcription factors. The altered expression of transcription factors upregulated the expression of anthocyanin biosynthetic genes such as anthocyanin‐3‐O‐glucosyl transferase and leucoanthocyanidin dioxygenase, while downregulated the expression of anthocyanin reductase and flavonoid 3′,5′,‐hydroxylase, which activated the anthocyanin biosynthesis pathway, ultimately leading to a significant increase in anthocyanin biosynthesis. This research work establishes a foundation for further research on the role of hormones in regulating anthocyanin biosynthesis in suspension‐cultured plant cells.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"78 1","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Bioengineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/bit.28913","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Hormones are effective in regulating plant metabolism and, therefore, are often used in plant cell culture to increase the yield of target products. This study investigated the effects of hormones on the growth and anthocyanin biosynthesis of suspension‐cultured red Cyclocarya paliurus cells. Additionally, the mechanism by which gibberellin induces anthocyanin biosynthesis was explored through multi‐omics integrated analysis and the assay of the dynamic changes in signaling molecule concentration. The results showed that the total anthocyanin content and yield of suspension‐cultured cells, when induced by 1.0 mg L⁻¹ Gibberellin A3 (GA3), experienced increases of 1.92‐ and 1.83‐fold, respectively. The application of exogenous GA3 activated the synthesis and transduction of four signaling molecules, that is, nitric oxide (NO), hydrogen peroxide (H2O2), salicylic acid (SA), and jasmonic acid (JA), in the cells and altered the expression patterns of transcription factors. The altered expression of transcription factors upregulated the expression of anthocyanin biosynthetic genes such as anthocyanin‐3‐O‐glucosyl transferase and leucoanthocyanidin dioxygenase, while downregulated the expression of anthocyanin reductase and flavonoid 3′,5′,‐hydroxylase, which activated the anthocyanin biosynthesis pathway, ultimately leading to a significant increase in anthocyanin biosynthesis. This research work establishes a foundation for further research on the role of hormones in regulating anthocyanin biosynthesis in suspension‐cultured plant cells.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

激素对柳花莲悬浮培养细胞生长及花青素合成的影响

激素在调节植物代谢方面是有效的，因此在植物细胞培养中经常使用激素来提高目标产物的产量。本研究研究了激素对悬浮培养的红柳花莲细胞生长和花青素合成的影响。此外，通过多组学综合分析和信号分子浓度动态变化分析，探讨了赤霉素诱导花青素生物合成的机制。结果表明，用1.0 mg L -⁻¹赤霉素A3 （giberellin A3, GA3）诱导后，悬浮培养细胞的总花青素含量和产量分别提高1.92倍和1.83倍。外源GA3的应用激活了细胞内一氧化氮（NO）、过氧化氢（H2O2）、水杨酸（SA）和茉莉酸（JA）四种信号分子的合成和转导，并改变了转录因子的表达模式。转录因子表达的改变上调了花青素- 3‐O‐葡萄糖基转移酶和浅色花青素双加氧酶等花青素生物合成基因的表达，下调了花青素还原酶和类黄酮3′，5′，‐羟化酶的表达，激活了花青素生物合成途径，最终导致花青素生物合成显著增加。本研究为进一步研究激素对悬浮培养植物细胞花青素合成的调控作用奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

期刊介绍： Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.