Color-Coded Traffic Signal Method Combined with Nanodiamond Quantum Sensing for Accurate miRNA Detection.

IF 8.2 1区化学 Q1 CHEMISTRY, ANALYTICAL ACS Sensors Pub Date : 2024-12-23 DOI:10.1021/acssensors.4c02511

Wei Wang, Youqiang Xing, Lei Liu, Min Wu, Peng Huang, Bingjue Li, Ze Wu

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引用次数: 0

Abstract

Background noise interferes with the accurate detection of early tumor biomarkers. This study introduces a method that effectively reduces background noise to enhance detection accuracy by combining a color-coded signaling approach with the unique fluorescent properties and room-temperature tunable quantum spin characteristics of fluorescent diamonds (FNDs) with nitrogen-vacancy centers. In this approach, a red signal indicates the presence of the target analyte within the spectral region, a green signal indicates its absence, and a yellow signal indicates the need for further analysis using FNDs' quantum spin properties for optical detection magnetic resonance (ODMR) to distinguish the FND signal from background noise. Preliminary results demonstrate that this method enables the detection of breast cancer-related miRNA-21 and miRNA-96 concentrations as low as 1 fM within a 100 × 100 μm² area, achieving single-molecule detection capability. This method is suitable for accurate biomarker identification and detection under high-background fluorescence conditions.

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来源期刊

ACS Sensors Chemical Engineering-Bioengineering

CiteScore

14.50

自引率

3.40%

发文量

372

期刊介绍： ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.