Implications of cyantraniliprole sublethal doses on the population dynamics and gene expression of Aphis gossypii glover (Hemiptera: Aphididae).

Abstract

Cyantraniliprole (CYA), widely recognized as a highly effective solution, is widely used in pest management. It has been broadly utilized to manage diverse pests, among which Aphis gossypii Glover (Hemiptera: Aphididae) is a prominent agricultural pest that leads to significant crop damage worldwide. Studies suggest that the sublethal effect of insecticides might contribute to the resurgence of A. gossypii. Therefore, in this study, A. gossypii were exposed to sublethal doses of CYA (LC₁₅ and LC₃₀ values of 1.43 and 2.93 mg/L, respectively) for 48 h then employed life table parameters and RT-qPCR were used to estimate the sublethal and cross-generational impacts. Treatments with sublethal doses of CYA notably reduced the survival and reproduction of the F₀A. gossypii and CYA at LC₃₀ significantly increased the fecundity and population growth parameters (R₀, r, λ, and GRR) of F₁ and reduced in the pre-adult stage. Furthermore, gene expression analysis indicated a significant downregulation of juvenile hormone-binding protein (JHBP) in F₀. Conversely, the F₁ generation exhibited an upregulation of vitellogenin (Vg), insulin receptor substrate 1 (InS1), ecdysone receptor (EcR), and ultraspiracle protein (USP). The funding not only enhance the comprehension of the sublethal effects of CYA on A. gossypii but also provide valuable guidance for the effective utilization of insecticides in managing the pest.