An aptamer sensor based on AgNPs@MOF for surface-enhanced Raman spectroscopy detection of sulfadimethoxine in food

IF 5.3 2区 化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-12-24 DOI:10.1007/s00604-024-06897-x
Zhanye Yang, Nan Zhang, Haiyang Lv, Xinge Ju, Yanhua Chen, Ziwei Zhang, Yuan Tian, Bing Zhao
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Abstract

A highly sensitive aptamer sensor (aptasensor) is proposed based on metal–organic frameworks-silver nanoparticles (AgNPs@MOF) to detect sulfadimethoxine (SDM) by surface-enhanced Raman spectroscopy (SERS). AgNPs@MOF with SERS activity was successfully fabricated by synthesizing AgNPs in situ on the surface of MIL-101(Fe), and SDM aptamer and Raman reporter 4-aminophenthiophenol (4-ATP) were selected as specific recognition elements and signal probes, respectively. When SDM was absent, the SDM aptamers were effectively adsorbed on the surface of AgNPs@MOF, thus keeping AgNPs@MOF in a dispersed state, resulting in a weakened SERS signal of 4-ATP. In the presence of SDM, the combination of SDM and aptamer formed a rigid hairpin SDM-aptamer complex, which bound less to AgNPs@MOF. Therefore, fewer aptamers were adsorbed on AgNPs@MOF, which exposed more hot spots, resulting in an enhanced SERS signal of 4-ATP. The aptasensor had good selectivity and sensitivity towards SDM and a good linear relationship between SERS intensity and SDM concentration in the range 6.00–150.00 ng/mL, with the limit of detection as low as 2.73 ng/mL. Further application to honey and chicken samples spiked with SDM resulted in satisfactory recoveries, and the aptasensor showed good stability and reproducibility in real samples. The aptasensor based on AgNPs@MOF was proposed for the first time to detect trace SDM by SERS, which provided a favorable way to develop various sensing platforms for antibiotic detection in food safety.

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来源期刊
Microchimica Acta
Microchimica Acta 化学-分析化学
CiteScore
9.80
自引率
5.30%
发文量
410
审稿时长
2.7 months
期刊介绍: As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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