Extracellular vesicles support increased expansion of mesenchymal stromal cells on fetal membrane-derived decellularized extracellular matrix.

IF 3.2 3区 生物学 Q3 CELL BIOLOGY Cell and Tissue Research Pub Date : 2024-12-24 DOI:10.1007/s00441-024-03946-y
Aida Shakouri-Motlagh, Andrea J O'Connor, Shaun P Brennecke, Daniel E Heath, Bill Kalionis
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Abstract

Decidual mesenchymal stromal cells (DMSC) were the source of extracellular vesicles (DMSC_EV). The xCELLigence real-time cell growth assay revealed increasing concentrations of EVs decreased DMSC attachment in the early growth phase but stimulated DMSC proliferation at day 7 when grown on tissue culture plastic (TCP). DMSC attachment and proliferation varied depending on the growth surface and DMSC_EV supplementation. DMSC attachment increased on decellularized and solubilized amniotic (s-dAM) whether or not EVs were added. Only Matrigel substrate increased DMSC attachment with added EVs. The addition of EVs increased DMSC proliferation only on the s-dAM substrate. DMSCs were more motile on s-dAM and decellularized and solubilized chorionic (s-dCM) membranes following EV addition. The osteogenic potential of DMSCs was improved on s-dAM substrates when supplanted with EVs. Finally, the levels of reactive oxygen species in DMSCs varied depending on the substrate but not on added EVs. We show that the addition of in vitro EVs isolated from the source being expanded (i.e., DMSCs) and the presence of ECM improve DMSC behaviours during ex vivo expansion. The inclusion of two key components of the MSC niche, EVs and ECM, benefitted the ex vivo expansion of MSCs. Added in vitro EVs increased the proliferation of DMSCs when grown on s-dAM but not on s-dCM, whereas they improved DMSC mobility on both surfaces. Testing different ECMs could be used to promote specific desired characteristics of DMSCs, and different combinations of EVs and ECM may enhance desirable MSC characteristics for specific therapeutic settings.

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细胞外囊泡支持胚胎膜来源的脱细胞细胞外基质上间充质间质细胞的增加。
细胞外囊泡(DMSC_EV)主要来源于蜕膜间充质基质细胞(DMSC)。xCELLigence实时细胞生长试验显示,在生长早期,ev浓度增加会降低DMSC的附着,但在组织培养塑料(TCP)上生长第7天,会刺激DMSC的增殖。DMSC的附着和增殖取决于生长表面和DMSC_EV的添加量。无论是否添加ev,脱细胞和溶解羊膜(s-dAM)上的DMSC附着均增加。随着ev的增加,只有基质增加了DMSC的附着。ev的加入只增加了s-dAM底物上DMSC的增殖。添加EV后,DMSCs在s-dAM和脱细胞和溶解的绒毛膜(s-dCM)膜上具有更强的运动性。用ev替代s-dAM基质后,DMSCs的成骨潜能得到改善。最后,DMSCs中的活性氧水平随底物而变化,但与添加的ev无关。我们发现,添加从正在扩增的源(即DMSCs)分离的体外ev和ECM的存在改善了DMSC在体外扩增过程中的行为。包括MSC生态位的两个关键组成部分,EVs和ECM,有利于MSC的体外扩增。体外添加ev可促进DMSCs在s-dAM表面的增殖,但对s-dCM表面的增殖无促进作用。测试不同的ECM可用于促进DMSCs的特定所需特性,并且EVs和ECM的不同组合可以增强特定治疗环境中理想的MSC特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell and Tissue Research
Cell and Tissue Research 生物-细胞生物学
CiteScore
7.00
自引率
2.80%
发文量
142
审稿时长
1 months
期刊介绍: The journal publishes regular articles and reviews in the areas of molecular, cell, and supracellular biology. In particular, the journal intends to provide a forum for publishing data that analyze the supracellular, integrative actions of gene products and their impact on the formation of tissue structure and function. Submission of papers with an emphasis on structure-function relationships as revealed by recombinant molecular technologies is especially encouraged. Areas of research with a long-standing tradition of publishing in Cell & Tissue Research include: - neurobiology - neuroendocrinology - endocrinology - reproductive biology - skeletal and immune systems - development - stem cells - muscle biology.
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