Inhibition of shoot branching in Arabidopsis by the artificially produced canonical-strigolactone.

Abstract

Strigolactones (SLs) are apocarotenoid plant hormones that regulate shoot branching. The natural SLs can be divided into two groups, canonical and non-canonical SLs according to those chemical structures. In a model plant, Arabidopsis thaliana, it has been thought to produce only non-canonical SLs. Moreover, in rice, it was suggested that canonical-SL such as 4-deoxyorobanchol (4DO) does not have a critical role in shoot branching inhibition. In this report, to understand the potential of canonical-SL in shoot branching inhibition pathway in Arabidopsis, SL biosynthetic genes involved in canonical-SL production in other plant species were individually expressed in Arabidopsis. Our data clearly demonstrate that 5-deoxystrigol, but not 4DO, can inhibit shoot branching in Arabidopsis. Moreover, the results confirmed the important role of CLA methyltransferase (CLAMT) in shoot branching inhibition pathway in Arabidopsis.