A de novo Gene Promotes Seed Germination Under Drought Stress in Arabidopsis.

IF 11 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular biology and evolution Pub Date : 2025-01-06 DOI:10.1093/molbev/msae262
Guang-Teng Jin, Yong-Chao Xu, Xing-Hui Hou, Juan Jiang, Xin-Xin Li, Jia-Hui Xiao, Yu-Tao Bian, Yan-Bo Gong, Ming-Yu Wang, Zhi-Qin Zhang, Yong E Zhang, Wang-Sheng Zhu, Yong-Xiu Liu, Ya-Long Guo
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Abstract

The origin of genes from noncoding sequences is a long-term and fundamental biological question. However, how de novo genes originate and integrate into the existing pathways to regulate phenotypic variations is largely unknown. Here, we selected 7 genes from 782 de novo genes for functional exploration based on transcriptional and translational evidence. Subsequently, we revealed that Sun Wu-Kong (SWK), a de novo gene that originated from a noncoding sequence in Arabidopsis thaliana, plays a role in seed germination under osmotic stress. SWK is primarily expressed in dry seed, imbibing seed and silique. SWK can be fully translated into an 8 kDa protein, which is mainly located in the nucleus. Intriguingly, SWK was integrated into an extant pathway of hydrogen peroxide content (folate synthesis pathway) via the upstream gene cytHPPK/DHPS, an Arabidopsis-specific gene that originated from the duplication of mitHPPK/DHPS, and downstream gene GSTF9, to improve seed germination in osmotic stress. In addition, we demonstrated that the presence of SWK may be associated with drought tolerance in natural populations of Arabidopsis. Overall, our study highlights how a de novo gene originated and integrated into the existing pathways to regulate stress adaptation.

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一个新生基因促进拟南芥在干旱胁迫下的种子萌发。
基因从非编码序列起源是一个长期和基本的生物学问题。然而,新生基因如何起源并整合到现有的调节表型变异的途径在很大程度上是未知的。在这里,我们从782个新生基因中选择了7个基因进行了基于转录和翻译证据的功能探索。随后,我们发现SWK是一个来自拟南芥非编码序列的新基因,在渗透胁迫下的种子萌发中起作用。SWK主要在干种子、吸干种子和硅油中表达。SWK可以完全翻译成一个8kda的蛋白,该蛋白主要位于细胞核中。有趣的是,SWK通过上游基因cytHPPK/DHPS和下游基因GSTF9整合到现有的过氧化氢含量途径(叶酸合成途径)中,以改善渗透胁迫下种子的萌发。cytHPPK/DHPS是拟南芥特有的基因,起源于mitHPPK/DHPS的重复。此外,我们还证明了SWK的存在可能与拟南芥自然种群的耐旱性有关。总的来说,我们的研究强调了一个新生基因如何起源并整合到现有的调节应激适应的途径中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular biology and evolution
Molecular biology and evolution 生物-进化生物学
CiteScore
19.70
自引率
3.70%
发文量
257
审稿时长
1 months
期刊介绍: Molecular Biology and Evolution Journal Overview: Publishes research at the interface of molecular (including genomics) and evolutionary biology Considers manuscripts containing patterns, processes, and predictions at all levels of organization: population, taxonomic, functional, and phenotypic Interested in fundamental discoveries, new and improved methods, resources, technologies, and theories advancing evolutionary research Publishes balanced reviews of recent developments in genome evolution and forward-looking perspectives suggesting future directions in molecular evolution applications.
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