Fluorescent microsphere-based strip for sensitive and quantitative detection of etomidate and metomidate†

IF 3.6 3区化学 Q2 CHEMISTRY, ANALYTICAL Analyst Pub Date : 2024-12-26 DOI:10.1039/D4AN01213E

Qingqing Liu, Xinxin Xu, Liqiang Liu, Aihua Qu, Chuanlai Xu and Hua Kuang

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Abstract

In this research, we fabricated a sensitive monoclonal antibody (mAb) 2C3 that targeted etomidate (ET) and metomidate (MT) to establish a lateral-flow immunoassay (LFIA) that incorporated fluorescent microsphere sensors, enabling both the qualitative and quantitative detection of ET and MT within 10 min. Analysis indicated that the visual colorimetric values for ET and MT in water samples were 0.3 μg kg⁻¹, respectively, with quantitative detection ranges of 0.08 to 1.31 μg kg⁻¹ and 0.08 to 2.21 μg kg⁻¹, respectively. The visual colorimetric values for ET and MT in urine samples were 0.3 and 1 μg kg⁻¹, respectively, with quantitative detection ranges of 0.12 to 3.13 μg kg⁻¹ and 0.09 to 2.98, respectively, while for ET and MT in serum samples were 3 μg kg⁻¹, with quantitative detection ranges of 0.15 to 21.2 μg kg⁻¹ and 0.08 to 13.8 μg kg⁻¹, respectively. Recovery tests and detection were conducted in water, urine and serum samples, validating the reliability of this method in clinical samples, consistent with those obtained from LC-MS/MS. Collectively, our novel LFIA provides a promising option for rapid on-site detection of ET and MT.

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荧光微球试纸灵敏定量检测依托咪酯和甲咪酯

在这项研究中，我们利用一种新的半抗原产生了一种针对依托咪酯（ET）和美托咪酯（MT）的敏感单克隆抗体（mAb） 2C3。我们利用该单抗建立了一种结合荧光微球传感器的侧流免疫分析法（LFIA），可以在10分钟内对ET和MT进行定性和定量检测。分析表明，水样中ET和MT的视觉比色值分别为0.3µg/kg，定量检测范围分别为0.08 ~ 1.31µg/kg和0.08 ~ 2.21µg/kg。尿液样品中ET和MT的视觉比色值为0.3 ~ 1µg/kg，定量检测范围分别为0.12 ~ 3.13µg/kg和0.09 ~ 2.98。血清样品中ET和MT的视觉比色值为3µg/kg，定量检测范围分别为0.15 ~ 21.2µg/kg和0.08 ~ 13.8µg/kg。在水、尿、血清样品中进行了回收率试验和检测，验证了该方法在实际临床样品中的可靠性。总的来说，我们的新型LFIA为快速现场检测ET和MT提供了一个有前途的选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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