Interplay between tobacco curly shoot virus vsiRNA24 and triosephosphate isomerase: implications for Nicotiana benthamiana viral defense

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-12-25 DOI:10.1111/nph.20359

Rui Wu, Gentu Wu, Muyao He, Haolan Zhang, Xinyi Shen, Qian Huang, Jiying Li, Menglin Wu, Hussein Ghanem, Mingjun Li, Ling Qing

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Abstract

Virus-derived small interfering RNAs (vsiRNAs) play an important role in viral infection by regulating the expression of host genes. At present, research on the regulation of plant primary metabolic pathways by vsiRNAs is very limited.
TvsiRNA24 derived from tobacco curly shoot virus (TbCSV) was amplified by reverse transcription polymerase chain reaction, and its target gene NbTPI (triosephosphate isomerase) was verified using reverse transcription quantitative polymerase chain reaction and GFP fluorescence observation. The effect of the interaction between TvsiRNA24 and NbTPI on TbCSV infection was analyzed by virus mediated, genetic transformation, western blotting, and quantitative detection.
The expression of TvsiRNA24 retards the growth of Nicotiana benthamiana and enhances TbCSV accumulation within N. benthamiana. The overexpression of NbTPI attenuates the accumulation of TbCSV, and the silencing of NbTPI leads to the growth retardation of N. benthamiana and intensifies symptoms post-TbCSV infection. Moreover, the expression of some genes related to photosynthesis, primary metabolism and immune response is regulated by NbTPI.
Our results unveil the specific role of TvsiRNA24-NbTPI in the pathogenicity of TbCSV, resulting in hindrance to plant growth and facilitation of viral infection. The identification of this regulatory pathway provides valuable insights that can be utilized to devise novel antiviral approaches targeting the reduction of viral pathogenicity.

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烟草卷枝病毒vsirna - 24与三磷酸异构体酶的相互作用：对烟叶病毒防御的影响

病毒源性小干扰rna （vsirna）通过调控宿主基因的表达在病毒感染中发挥重要作用。目前，关于vsirna调控植物初级代谢途径的研究非常有限。利用逆转录聚合酶链反应扩增烟草卷枝病毒（TbCSV）的TvsiRNA24，并利用逆转录定量聚合酶链反应和GFP荧光观察对其靶基因NbTPI（三磷酸异构酶）进行了验证。通过病毒介导、基因转化、western blotting和定量检测分析了TvsiRNA24与NbTPI互作对TbCSV感染的影响。TvsiRNA24的表达抑制了本烟菌的生长，促进了本烟菌TbCSV的积累。NbTPI的过表达会减弱TbCSV的积累，而NbTPI的沉默会导致benthamiana生长迟缓，并加剧TbCSV感染后的症状。此外，NbTPI还能调控光合作用、初级代谢和免疫应答相关基因的表达。我们的研究结果揭示了TvsiRNA24‐NbTPI在TbCSV致病性中的特定作用，导致阻碍植物生长和促进病毒感染。这种调控途径的鉴定提供了有价值的见解，可用于设计新的抗病毒方法，以降低病毒的致病性。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.