Rapid evaluation of hepatocellular carcinoma by detecting plasma exosomes with time-resolved fluorescence immunochromatographic test strips

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-12-28 DOI:10.1007/s00604-024-06903-2

Jiaming Li, Jianfen Su, Minghui Li, Yaofen Wu, Huiqiang Chen, Xihua Fu, Hongliang Yao, Jinping Chen, Yuntao Liu, Jie Zan

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Abstract

Time-resolved fluorescence immunochromatographic test strips (TRFIS) was developed for the rapid detection of hepatocellular carcinoma (HCC)-specific plasma exosomes (hExos) by targeting the hExo-surface membrane protein glypican-3 (GPC3). The GPC3-TRFIS could directly detect plasma exosomes without the isolation and purification process, and the whole immunoassay could be completed within 15 min. The visual detection limit of GPC3-TRFIS was 3.44 × 10^9 particles/mL, with a minimum detection limit of 1.8 × 10^9 particles/mL. For analysis of the clinical HCC samples, GPC3-TRFIS shows high specificity for detection of hExo, and was nearly unreactive for healthy donors’ samples. GPC3-TRFIS was able to efficiently distinguish HCC patients (19 cases) from healthy donors (19 cases). Overall, the developed TRFIS offers the benefits of high sensitivity, simple operation, and no need of large precision instruments and professional technical personnel for rapid detection of plasma hExos, and supplies a novel approach for early screening of HCC.

Graphical Abstract

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用时间分辨荧光免疫层析试纸条检测血浆外泌体对肝癌的快速评价

以肝细胞癌（HCC）特异性血浆外泌体（hExos）表面膜蛋白glypican-3 （GPC3）为靶点，建立了时间分辨荧光免疫层析试纸条（TRFIS），用于快速检测HCC特异性血浆外泌体（hExos）。GPC3-TRFIS可直接检测血浆外泌体，无需分离纯化过程，整个免疫分析可在15 min内完成。GPC3-TRFIS的视觉检出限为3.44 × 10^9颗粒/mL，最低检出限为1.8 × 10^9颗粒/mL。对于临床HCC样本的分析，GPC3-TRFIS对hExo的检测具有很高的特异性，而对健康供者的样本几乎没有反应。GPC3-TRFIS能够有效区分HCC患者（19例）和健康供体（19例）。总之，所研制的TRFIS具有灵敏度高、操作简单、无需大型精密仪器和专业技术人员即可快速检测血浆hExos的优点，为HCC的早期筛查提供了一种新的方法。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.