DETECTION OF MECA AND NUC GENES OF MULTI-DRUG RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT CLINICAL SAMPLES.

Q4 Medicine Georgian medical news Pub Date : 2024-10-01

S Abood, L Shihab, G Abed, Th Azzawi, A Abood

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Abstract

Background: During this study, six isolates of multiple antibiotic resistant Staphylococcus aureus bacteria were obtained from different clinical specimens (burn swabs, urinary tract infections, wound swabs): three isolates from burns, two isolates from urinary tract infections, and one isolate from wound swabs. They were obtained from private laboratories in Baghdad from 1/1/2023 to 3/15/2023.

Method: The diagnosis of these isolates was confirmed using the Vitek2 device. A susceptibility test was conducted on ten antibiotics, and S. aureus bacteria showed resistance to most antibiotics, polymerase chain reaction was done to mecA and Nuc gene by conventional PCR.

Results: The results of the molecular detection of the MecA gene showed that all isolates of multi-drug-resistant S. aureus possess this gene. In contrast, the results of the molecular detection of the nuc gene showed that only isolates No. 1 and No. 4 carry this gene, while the rest of the isolates do not carry this gene.

Conclusion: S. aureus are resistant to antibiotics because they possess resistance genes such as the mecA gene.

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不同临床样品多重耐药金黄色葡萄球菌meca和nuc基因的检测。

背景：本研究从不同临床标本（烧伤拭子、尿路感染、创面拭子）中分离到6株多重耐药金黄色葡萄球菌：烧伤3株，尿路感染2株，创面拭子1株。它们是在2023年1月1日至2023年3月15日期间从巴格达的私人实验室获得的。方法：采用Vitek2仪器对分离株进行诊断。对10种抗生素进行药敏试验，金黄色葡萄球菌对大部分抗生素均有耐药性，采用常规PCR对mecA和Nuc基因进行聚合酶链反应。结果：多重耐药金黄色葡萄球菌MecA基因分子检测结果显示，所有分离株均含有该基因。相比之下，nuc基因的分子检测结果显示，只有1号和4号分离株携带该基因，其余分离株均不携带该基因。结论：金黄色葡萄球菌具有耐药基因，如mecA基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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