Ectopic expression of LONELY GUY7 in epidermis of internodal segments for de novo shoot regeneration without phytohormone treatment in ipecac.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2025-01-01 DOI:10.1111/ppl.70023
Karin Okazaki, Wataru Katano, Kyomi Shibata, Masashi Asahina, Kazuko Koshiba-Takeuchi, Koichiro Shimomura, Mikihisa Umehara
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Abstract

In many plant species, the application of exogenous phytohormones is crucial for initiating de novo shoot regeneration. However, ipecac [Carapichea ipecacuanha (Brot) L. Andersson] has a unique ability to develop adventitious shoots on the epidermis of internodal segments without phytohormone treatment. This characteristic allows us to evaluate the effects of endogenous phytohormones in this species. Here, we showed that the presence of the pith, including vascular bundles in the internodal segment, is required to activate both endogenous cytokinin (CK) biosynthesis and adventitious shoot formation. Adventitious shoots were mainly formed in the apical region of internodal segments, where the CK biosynthesis genes ISOPENTENYL TRANSFERASE 3 (CiIPT3) and LONELY GUY 7 (CiLOG7) were spontaneously upregulated in the early culture stage on phytohormone-free medium. In addition, CiIPT3 and CiLOG7 were respectively expressed in the pith and the epidermis of the internodal segments. The expression of CiLOG7 was localized as several spots on the epidermis. These findings suggest that CK precursors are generated in the pith, transferred to the epidermis, and then converted into active CKs, facilitating adventitious shoot formation on the epidermis. Conversely, auxin levels rapidly decreased during culture and remained low in the region of shoot formation. Auxin is transferred to the basal region of internodal segments, and strongly suppressed the CiLOG7 expression and decreased the CK levels. Thus, we conclude that the ectopic expression of CiLOG7 in the epidermis of internodal segments contributes to de novo shoot regeneration in ipecac.

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在根茎节间节段表皮中LONELY GUY7的异位表达对根茎再生的影响。
在许多植物物种中,外源植物激素的应用是启动新生芽再生的关键。然而,根根[Carapichea ipecacuanha (Brot) L. Andersson]在没有植物激素处理的情况下具有在节间节表皮上发育不定芽的独特能力。这一特性使我们能够评估内源植物激素对该物种的影响。在这里,我们发现髓的存在,包括节间节的维管束,是激活内源性细胞分裂素(CK)生物合成和不定芽形成所必需的。不定芽主要在节间节的顶端区域形成,CK生物合成基因异opentenyl TRANSFERASE 3 (CiIPT3)和LONELY GUY 7 (CiLOG7)在培养早期在无激素培养基上自发上调。此外,CiIPT3和CiLOG7分别在节间节的髓和表皮表达。CiLOG7的表达定位于表皮上的几个点。这些结果表明,CK前体在髓中产生,转移到表皮,然后转化为活性CK,促进表皮上的不定根形成。相反,生长素水平在培养过程中迅速下降,并在芽形成区域保持较低水平。生长素转移到结间节基底区,强烈抑制CiLOG7的表达,降低CK水平。因此,我们得出结论,在根茎节段表皮中,CiLOG7的异位表达有助于根茎新生。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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