Increased Expression of MST1 in Patients With Epilepsy and in a Rat Model of Epilepsy.

IF 1.6 4区 医学 Q4 NEUROSCIENCES Synapse Pub Date : 2025-01-01 DOI:10.1002/syn.70002
Di Xia, Linming Zhang, Rong Mei, Chunhua Wu, Yan Liu, Hongyu Chen, Ling Chen
{"title":"Increased Expression of MST1 in Patients With Epilepsy and in a Rat Model of Epilepsy.","authors":"Di Xia, Linming Zhang, Rong Mei, Chunhua Wu, Yan Liu, Hongyu Chen, Ling Chen","doi":"10.1002/syn.70002","DOIUrl":null,"url":null,"abstract":"<p><p>Mammalian sterile20-like kinase 1 (MST1), a serine/threonine kinase frequently expressed, has emerged as pivotal modulator of multiple physiological and pathological conditions such as cellular growth, programmed cell death, oxidative stress, neurodegeneration, inflammation, and synaptic plasticity in the central nervous system. Various neurological diseases are associated with the activation of MST1. Epilepsy is a severe neurological disorder characterized by abrupt abnormal electrical activity in the brain and recurring spontaneous seizures. The most common pathological discoveries in patients and animal models with epilepsy are neuronal death, inflammation, neurodegeneration, neurogenesis, and axonal regrowth. The purpose of this study was to assess the levels of MST1 in serum and cerebrospinal fluid (CSF) specimens obtained from individuals diagnosed with epilepsy. In addition, it aimed to explore the expression pattern of MST1 in brain tissues of epileptic rats. We used enzyme-linked immunosorbent assay to measure the levels of CSF and serum MST1 in 10 epilepsy patients and 9 control patients. After creation of epilepsy models with healthy male Sprague-Dawley rats using lithium and pilocarpine, the expression of MST1 in the temporal cortex and hippocampus was evaluated at different time points (6 h, 24 h, 3 days, 7 days, 14 days, and 30 days after seizures) using immunofluorescence, immunohistochemistry, and Western blotting. In patients with epilepsy, the levels of CSF-MST1 were elevated (593.90 ± 16.28 vs. 560.40 ± 19.42 pg/mL, p < 0.05) compared to the control group. Accordingly, the serum-MST1 levels were 583.40 ± 19.70 pg/mL in the epilepsy group and 555.70 ± 20.14 pg/mL in the control group, demonstrating a statistically significant distinction (p < 0.05). Levels of MST1 in CSF and serum could be of diagnostic help. Neuronal apoptosis in temporal cortex and hippocampus of epileptic rats was detected using terminal deoxynucleotidyl transferase dUTP nick end labeling staining. MST1 was expressed in the neuronal membrane and cytoplasm of the temporal cortex and hippocampus. The expression of MST1 increased after seizures, showing a relatively high level within 30 days and reaching its highest point on the seventh day after status epilepticus. The findings of this study indicate that the increased expression of MST1 protein in patients with epilepsy and epileptic rats might play a role in the development of epilepsy.</p>","PeriodicalId":22131,"journal":{"name":"Synapse","volume":"79 1","pages":"e70002"},"PeriodicalIF":1.6000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Synapse","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/syn.70002","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Mammalian sterile20-like kinase 1 (MST1), a serine/threonine kinase frequently expressed, has emerged as pivotal modulator of multiple physiological and pathological conditions such as cellular growth, programmed cell death, oxidative stress, neurodegeneration, inflammation, and synaptic plasticity in the central nervous system. Various neurological diseases are associated with the activation of MST1. Epilepsy is a severe neurological disorder characterized by abrupt abnormal electrical activity in the brain and recurring spontaneous seizures. The most common pathological discoveries in patients and animal models with epilepsy are neuronal death, inflammation, neurodegeneration, neurogenesis, and axonal regrowth. The purpose of this study was to assess the levels of MST1 in serum and cerebrospinal fluid (CSF) specimens obtained from individuals diagnosed with epilepsy. In addition, it aimed to explore the expression pattern of MST1 in brain tissues of epileptic rats. We used enzyme-linked immunosorbent assay to measure the levels of CSF and serum MST1 in 10 epilepsy patients and 9 control patients. After creation of epilepsy models with healthy male Sprague-Dawley rats using lithium and pilocarpine, the expression of MST1 in the temporal cortex and hippocampus was evaluated at different time points (6 h, 24 h, 3 days, 7 days, 14 days, and 30 days after seizures) using immunofluorescence, immunohistochemistry, and Western blotting. In patients with epilepsy, the levels of CSF-MST1 were elevated (593.90 ± 16.28 vs. 560.40 ± 19.42 pg/mL, p < 0.05) compared to the control group. Accordingly, the serum-MST1 levels were 583.40 ± 19.70 pg/mL in the epilepsy group and 555.70 ± 20.14 pg/mL in the control group, demonstrating a statistically significant distinction (p < 0.05). Levels of MST1 in CSF and serum could be of diagnostic help. Neuronal apoptosis in temporal cortex and hippocampus of epileptic rats was detected using terminal deoxynucleotidyl transferase dUTP nick end labeling staining. MST1 was expressed in the neuronal membrane and cytoplasm of the temporal cortex and hippocampus. The expression of MST1 increased after seizures, showing a relatively high level within 30 days and reaching its highest point on the seventh day after status epilepticus. The findings of this study indicate that the increased expression of MST1 protein in patients with epilepsy and epileptic rats might play a role in the development of epilepsy.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
MST1在癫痫患者和癫痫大鼠模型中的表达增加。
哺乳动物sterile20样激酶1 (MST1)是一种频繁表达的丝氨酸/苏氨酸激酶,已成为多种生理和病理条件的关键调节剂,如细胞生长、细胞程序性死亡、氧化应激、神经退行性变、炎症和中枢神经系统突触可塑性。多种神经系统疾病与MST1的激活有关。癫痫是一种严重的神经系统疾病,其特征是大脑突然异常的电活动和反复的自发发作。在癫痫患者和动物模型中最常见的病理发现是神经元死亡、炎症、神经变性、神经发生和轴突再生。本研究的目的是评估癫痫患者血清和脑脊液(CSF)标本中MST1的水平。同时,探讨MST1在癫痫大鼠脑组织中的表达规律。采用酶联免疫吸附法测定10例癫痫患者和9例对照患者脑脊液和血清MST1水平。用锂和匹罗卡品建立健康雄性Sprague-Dawley大鼠癫痫模型后,采用免疫荧光、免疫组织化学和Western blotting检测不同时间点(癫痫发作后6 h、24 h、3 d、7 d、14 d和30 d)颞叶皮质和海马中MST1的表达。在癫痫患者中,CSF-MST1水平升高(593.90±16.28比560.40±19.42 pg/mL, p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Synapse
Synapse 医学-神经科学
CiteScore
3.80
自引率
0.00%
发文量
38
审稿时长
4-8 weeks
期刊介绍: SYNAPSE publishes articles concerned with all aspects of synaptic structure and function. This includes neurotransmitters, neuropeptides, neuromodulators, receptors, gap junctions, metabolism, plasticity, circuitry, mathematical modeling, ion channels, patch recording, single unit recording, development, behavior, pathology, toxicology, etc.
期刊最新文献
Synaptosomal-Associated Protein 25 kDA (SNAP-25) Levels in Cerebrospinal Fluid: Implications for Alzheimer's Disease Diagnosis and Monitoring. Hippocampal Granule Cells Downregulate Their GABAergic Phenotype and Deactivate Its Activity-Dependent Reinduction in Culture Conditions. Cellular-Resolution and Bulk-Fluorescence Recordings of Calcium Activity Yield Reciprocal Readouts of In Vivo Drug Efficacy. Increased Expression of MST1 in Patients With Epilepsy and in a Rat Model of Epilepsy. Synergistic Anxiolytic Effects of Linalool and Sesamol Co-Treatment on Swiss Albino Mice: A Potential GABAergic Intervention.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1